| Description | Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.
Configuration changes in minutes -
Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.
Increased dynamic range and sensitivity -
Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.
Other features and benefits include: - Get automatic Z-height optimization
- Eliminate manual gain adjustment
- Tailor your data analysis
... Read More | The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver flexible, scalable solutions for absorbance, fluorescence, and luminescence assays—with advanced capabilities for evolving research needs. The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver flexible, scalable solutions for absorbance, fluorescence, and luminescence assays—with advanced capabilities for evolving research needs. The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and temperature control, while the iD5e reader adds enhanced detection modes including time-resolved fluorescence (TRF) and tunable fluorescence polarization (FP) and can be expanded to include TR-FRET, HTRF, BRET and western blot. Both readers support on board fluidics with optional dual injectors.
For live-cell assays and microbial growth experiments, environmental control for CO2/O2 regulation and advanced shaking are available to maintain ideal growth conditions. Data acquisition and analysis are driven by SoftMax® Pro Software—the most cited microplate reader software in the industry.
Features:
- Supports diverse assay needs -
Perform ELISA, nucleic acid and protein quantitation, cell viability, microbial assays, western blot, and more.
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Simplified setup & analysis -
Over 200 preconfigured protocols and intuitive setup tools help streamline data acquisition and analysis—backed by the industry’s most cited microplate reader software.
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Flash Assays Icon -
Fast, consistent mixing for high-performance flash assays
Dual injectors available for fast kinetic assays with SmartInject® Technology, ensuring complete reagent mixing in every well for high-precision with 10 µL dead volume.
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Superior hybrid optical system-
The high-efficiency monochromator and filter-based optical system, combined with an ultra-cooled photomultiplier tube (PMT), minimizes noise while increasing sensitivity, selectivity, and dynamic range.
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Live cell assays-
Advanced shaking and user-installable gas mixer (CO2/O2) that support ideal environments for cell-based and microbial growth experiments are available as optional upgrades.
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Automatic filter identification-
Automatically identify filter sets using NFC functionality, simplifying workflows and ensuring accuracy and reliability.
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Flexible temperature control-
Precise and intuitive temperature regulation—from 5°C above ambient conditions up to 66 °C—empowering your lab to perform a wide range of temperature-sensitive assays with confidence.
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Read height optimization (Z height)-
Z-height optimization automatically adjusts the detection height to maximize sensitivity and signal quality—while minimizing sample volume—ensuring reliable results even in low-volume or precious samples.
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Microvolume & cuvette options-
The SpectraDrop Micro-Volume Microplate for low-volume (2 to 4 µL) and SpectraCuvette™ Adapter Plate for cuvette-based applications eliminate the need for additional instrumentation.
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Personalized touchscreen control-
The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.
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Western blot capable-
The optional enhanced time-resolved fluorescence (TRF) detection module is optimal for quantitating as little as femtogram protein samples, providing improved sensitivity, extended signal stability and versatility to support a wide range of applications.
... Read More | The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter workflows including multiplexed TR-FRET and AlphaScreen® assays, live-cell kinetic studies, and cytotoxicity assays. With over 85,000 scientific citations and four decades of trusted performance and partnership in research and regulated labs, the SpectraMax i-Series gives you the flexibility to adapt as your needs evolve. Whether you're accelerating discovery, ensuring QC consistency, or preparing for regulatory compliance, SpectraMax readers—paired with powerful, GxP-ready SoftMax® Pro Software—offer a future-ready platform that grows with your lab.
Reliable ELISA & Protein Quantitation- Perform sandwich and competitive ELISAs with high sensitivity
- Tunable monochromators and hybrid optics for assay flexibility
- Ideal for cytokine profiling, biomarker validation, and QC labs
Live-Cell & Cell Viability Assays- Support fluorescence and luminescence-based viability assays
- Optional CO₂/O₂ control and advanced shaking for live-cell workflows
- Suitable for cytotoxicity, proliferation, and pathway analysis
Multiplexed & Advanced Detection Assays- Perform TRF, fluorescence polarization, and multiplexed assays (iD5e/i3x)
- Expand into TR-FRET, HTRF®, and western blot detection as needs grow
- Reduce instrument sprawl while future-proofing your lab
... Read More |
| Reading Modes | Endpoint reading, Kinetic reading, Well Area Scanning | Absorbance, Fluorescence (top/bottom read), Luminescence (top/bottom read), Time-Resolved Fluorescence (top/bottom read), Fluorescence Polarization (top/bottom read), HTRF (top/bottom read), HTRF | Absorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence (TRF), Fluorescence Polarization, TR-FRET, HTRF®, AlphaScreen® |