| Description | Flash and glow luminescence detection in an upgradeable platform.
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold Flash and glow luminescence detection in an upgradeable platform.
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring continuous monitoring during injection and measurement.
Reduced noise -
Ultra-fast photon counting technology amplifies the PMT signal with a fast pulse amplifier. This reduced noise capability enables a higher signal-to-noise ratio and low crosstalk.
PMT calibration -
The automatic PMT calibration method is based on readings of 4 internal LEDs at 395, 470, 527, and 570 nm. The manual PMT calibration method is based on readings of 4 wells from the assay.
Other features and benefits include: - Detect all samples
- Get 2-fold improvement in speed
- Automate injector workflows
... Read More | Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
Features : - White light and fluorescent imaging -
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green). - Data tools to accelerate research timelines -
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. - Quickly image a variety of plate formats and cell types -
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types. - Intelligent analysis with easy-to-use software -
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training. - High-resolution images -
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days. - Custom automation options -
The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research.
... Read More | Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes.
With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
User-configurable applications -
User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.
No-stain cell analysis -
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.
Multi-channel functionality -
Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.
Cellular pathway analysis -
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
Other features and benefits include: - Capture flash assays with ease
- Reduce background noise
- Increase fluorescence performance
... Read More | Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the SpectraMax® MiniMaxTM imaging cytometer option adds first-of-its-kind cellular imaging capability to a microplate reader. The upgradable option makes cytometry more accessible to a wider audience, providing researchers Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the SpectraMax® MiniMaxTM imaging cytometer option adds first-of-its-kind cellular imaging capability to a microplate reader. The upgradable option makes cytometry more accessible to a wider audience, providing researchers with cellular analysis capability without the need for investment in complex imaging systems. With brightfield and fluorescent imaging modes, the imaging cytometry option enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression.
Features - Simple workflow: Cell and whole-well assays in a small footprint
- More data: Multi-parametric cell-based assays with brightfield and fluorescent modes
- Familiar software: Set up, image and analyze cells in a snap using SoftMax® Pro Software
- Higher sensitivity: Use fewer and or less fluorescent cells compared to whole-well measurements
- Lower variance: Normalize readings with number of cells in a well
... Read More | Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.
Configuration changes in minutes -
Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.
Increased dynamic range and sensitivity -
Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.
Other features and benefits include: - Get automatic Z-height optimization
- Eliminate manual gain adjustment
- Tailor your data analysis
... Read More |