Microplate readers are a reliable choice for many labs looking for a fast assessment of enzymatic activity or nucleic acid and protein quantification. With applications in pharmaceuticals, biomanufacturing, and many more, these instruments can be used to detect absorbance, fluorescence, luminescence or a combination of those with a multimode meter. Fluorescence plate readers offer precise measurement of any fluorescence intensity assay.
Things to consider when purchasing a fluorescence plate reader include desired throughput, if a single or multimode plate reader would be needed for current or possibly future experiments, the types of assays being performed, ease-of-use, and price point.
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| Company | Agilent Technologies | Agilent Technologies |
| Item | Agilent BioTek Synergy LX Multimode Reader | Agilent BioTek Cytation 9 Cell Imaging Multimode Reader |
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| Catalog Number | Synergy LX | Cytation 9 |
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| Description | The Agilent BioTek Synergy LX multimode reader automates many common microplate assays. The high-quality optic design ensures excellent data in absorbance, fluorescence, and luminescence detection modes. This affordable system is ideal for many common assays including nucleic acid and protein The Agilent BioTek Synergy LX multimode reader automates many common microplate assays. The high-quality optic design ensures excellent data in absorbance, fluorescence, and luminescence detection modes. This affordable system is ideal for many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford, and cell viability. Gen6 data analysis software provides complete reader control and data analysis, and includes many pre-defined experiments, ready to customize for your assays. Synergy LX is available with a large touch screen user interface and onboard software for endpoint measurements and analysis.... Read More | The Agilent BioTek Cytation 9 cell imaging multimode reader is the next evolution in the Cytation portfolio, merging sophisticated hybrid microplate detection with both widefield inverted and upright microscopy. This modular, future-proofed platform is engineered to evolve with your research, The Agilent BioTek Cytation 9 cell imaging multimode reader is the next evolution in the Cytation portfolio, merging sophisticated hybrid microplate detection with both widefield inverted and upright microscopy. This modular, future-proofed platform is engineered to evolve with your research, offering a starting point of microscopy, multimode detection, or a powerful combination of both.
The advanced hybrid optics use variable bandwidth monochromators and high-performance filters to deliver unmatched sensitivity for demanding assays, such as Alpha and TR-FRET. For high-performance microscopy, a redesigned five-position filter carrier supports four imaging cubes alongside a dedicated laser autofocus module, ensuring rapid and consistent focus for complex three-dimensional (3D) biological samples.
To support sensitive live-cell kinetic studies, an optional integrated CO2/O2 gas control module and dual-reagent injectors provide precise environmental and liquid handling control. For long-term culturing of complex models, such as organoids and stem-cell-derived lineages, Cytation 9 integrates seamlessly with the Agilent BioTek BioSpa 8 automated incubator to provide essential humidity control. Whether used as a standalone unit or integrated into high-throughput automation, Cytation 9 is truly ready for any assay.
Features:
- Streamlined Discovery: A single, comprehensive platform that covers a diverse set of biochemical and cell-based assays.
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Richer Cellular Insights: Capture more data per well with an expanded image cube capacity and enhanced optics.
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Optimized Workflow Speed: Experience faster imaging throughput to move from raw samples to analyzed results more efficiently.
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Superior Kinetic Data: Software-controlled gas modules create a stable, monitored environment for live cell studies.
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Ready for Any Assay: The modular design grows with your lab, allowing you to add imaging modes as needs evolve.
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| Optical System | Monochromator or Filter-based | Inquire |
| Measuring Time | Absorbance - 12 s (96 wells), 23 s (384 wells)
Fluorescence intensity - 24 s (96 wells), 76 s (384 wells) | Inquire |
| Wavelength Range | 200 nm to 999 nm, in 1 nm increments (Absorbance)
320 to 700 nm (low noise PMT), 320 to 850 nm (red-shifted PMT) | Inquire |
| Plate Formats | 6 to 384-well plates | 6- to 1536-well plates, 35 and 60 mm cell culture dishes, T25 flasks, slides, hemocytometers, chamber slides |
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