High-performance liquid chromatography (HPLC) systems are used to analyze and/or purify liquid mixtures. Analytical HPLC systems are used in the food safety, pharmaceutical, and manufacturing industries, as well as in medical and research laboratories. Two of the most common types of analytical HPLC systems are normal phase HPLC and reversed phase HPLC. Normal phase uses a polar stationary phase and a non-polar mobile phase to separate components of a mixture, while reversed phase HPLC uses phases with the opposite polarities of normal phase HPLC. The sample liquid is mixed with the mobile phase and run through a column that contains the stationary phase. The way the sample moves through the column is the basis for the analysis.
Considerations for purchasing an Analytical HPLC System
To determine which analytic HPLC system is the best fit for your lab, decide which features will be needed. Multi-solvent or multi-column analysis, stationary phase pore size, possible pump pressures, possible flow rates, and number of wavelength detectors are all factors that can vary.
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JASCO
- Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer
- PU-4180: up to 10 mL/min (conventional to semi-prep, up to 10 mm I.D. column)
- 700 Bar (70 MPa)
JASCO
- UV-Vis, Fluorescence, PDA, Multi-Wavelength, Circular Dichroism Detectors
- PU-4580: 0.5 uL to 10.0 mL/min
- 50 MPa from 0.5 to 6 mL/min
35MPa up to 10mL/min
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