| Description | The majority of cell-free protein expression systems are generally derived from cell extracts, which usually contain nonspecific proteases and nucleases that have negative effects on protein synthesis. In addition, such unpredictable activities may also interfere with downstream assays. Completely The majority of cell-free protein expression systems are generally derived from cell extracts, which usually contain nonspecific proteases and nucleases that have negative effects on protein synthesis. In addition, such unpredictable activities may also interfere with downstream assays. Completely free from unwanted/uncharacterized cellular components, the PURE system introduces a much cleaner background. Magic™ PURE protein expression is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E.coli translation. The procedure can be performed in a single reaction tube from RNA transcription to protein synthesis. With minimal nuclease and protease activity, Magic™ Cell-Free PURE protein expression kit preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a single tube reaction. This in vitro cell-free expression system provides the benefits of microliter scale reactions and speed. With results available in a few hours, the kit saves valuable laboratory time and is ideal for high throughput technologies.... Read More | iNeuMito™ biosensor is a mitochondrial calcium flux sensor. These cells are engineered to express iNeuMito™ biosensor, which has been developed to use a highly sensitive fluorescence-based biosensor as a reporter gene to monitor mitochondrial calcium flux and cytotoxicity. Excitation = iNeuMito™ biosensor is a mitochondrial calcium flux sensor. These cells are engineered to express iNeuMito™ biosensor, which has been developed to use a highly sensitive fluorescence-based biosensor as a reporter gene to monitor mitochondrial calcium flux and cytotoxicity. Excitation = 610nm; emission = 625nm. The iNeuMito™ biosensor is introduced into the cell using a proprietary magnetic liposome-mediated method and expressed in the mitochondria (using a marker protein such as COX IV).
iNeuATP™, a fluorescent biosensor, has also been custom incorporated into the human iNeuRapid™ cell line. With iNeuATP™, biosensor assays provide fast kinetics (resolution in seconds) when measuring real-time cellular ATP metabolism and cytotoxicity. The analysis of cellular oxidative phosphorylation directly reflects the health and function of mitochondria. Excitation = 535nm; emission = 550nm.
Starting Materials: immortalized human cell line.... Read More | This product GTVCR-WQ1664MR is an effective transcription system suitable for a variety of research, including the in vitro translation, self-amplifying mRNA studies and protein expression. The system was driven by the T7 promoter to efficiently generate mRNA with 120 nt poly(A) tail ecoding This product GTVCR-WQ1664MR is an effective transcription system suitable for a variety of research, including the in vitro translation, self-amplifying mRNA studies and protein expression. The system was driven by the T7 promoter to efficiently generate mRNA with 120 nt poly(A) tail ecoding antibody which targeting EPHA3. The vector contains the self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon, which resulting the mRNA expression at a high level.... Read More | Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Some species of Lactobacillus used commonly as probiotics. They are most commonly used for diarrhea, including infectious diarrhea and diarrhea in people taking antibiotics. Some people also use lactobacillus for general digestion problems, irritable bowel syndrome; IBS, colic in infants, and many other conditions that involve the stomach and bowel.... Read More | This product is a 31 kDa recombinant human Clusterin protein Antigen with a N-terminal His6-ABP tag expressed in the E. coli. It is available for Antibody Competition. |