MALDI TOF mass spectrometers (matrix-assisted laser desorption/ionization/time-of-flight) are amongst the most popular mass spec instruments in biology. They combine two technologies, the “soft” MALDI ionization source and the TOF mass analyzer. The latter measures an ion’s mass-to-charge ratio (m/z) by measuring the time it takes to reach a detector. The former volatilizes and ionizes those molecules using a laser, a target, and an organic compound called a matrix. In MALDI, a sample (for instance, a collection of tryptic peptides) is mixed with matrix, spotted and dried (crystallized) on the surface of a metal target plate. Upon irradiation with laser light, the matrix absorbs the energy and converts it to heat, which then causes the sample to ionize and explode off the plate into the mass analyzer. MALDI TOF mass spectrometers come in two basic flavors, MALDI TOF MS and MALDI TOF/TOF MS; the latter enables tandem MS (MS/MS) studies.
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| Company | Shimadzu | Shimadzu | Shimadzu |
| Item | MALDImini-1 Compact MALDI Digital Ion Trap Mass Spectrometer | Axima Assurance MALDI Time of Flight Mass Spectrometer | OmegaTOF Benchtop High Mass MALDI-TOF Mass Spectrometer |
| Citations | | | |
| Catalog Number | MALDImini-1 | AXIMA Assurance | OmegaTOF |
| Price | | | |
| Ionization Method(s) | MALDI ion source | MALDI | MALDI |
| Mass Analyzer | Digital Ion Trap (DIT) | TOF | Time-of-Flight |
| Resolution | Inquire | >5000 FWHM | >5000 FWHM – ACTH 18-39 (m/z 2465) |
| Scanning Speed | Inquire | Inquire | Inquire |
| Mass Range | Inquire | 1 kDa to 500 kDa | m/z 1,000–1,500,000Da |
| Description | Inquire | An affordable, robust option for laboratories requiring routine manual or automated analysis of a wide variety of sample classes, the AXIMA Assurance provides high-quality, rapid MALDI mass spectra and an array of software tools for data processing and reporting. Incorporating a variable repetition An affordable, robust option for laboratories requiring routine manual or automated analysis of a wide variety of sample classes, the AXIMA Assurance provides high-quality, rapid MALDI mass spectra and an array of software tools for data processing and reporting. Incorporating a variable repetition rate 50Hz N2 laser, it is ideally suited for high-throughput QA/QC analysis of oligonucleotide/primers, synthetic peptides/proteins, polymers, and other small molecules. Features: High Sensitivity/Resolution:
A variable repetition rate 50 Hz N2 laser and advanced ion optics for enhanced ion transmission enable users to meet all sample throughput requirements. Pulsed extraction of ions from the MALDI source improves resolution, and enhanced calibration algorithms with easy-to-use software provide more accurate data. Positive and Negative Ion Modes Included as Standard:
Allows greater flexibility and extends the compound categories that may be analyzed. Patented Beam Blanker:
Removes unwanted low mass ions and prevents detector saturation. Unparalleled Flexibility:
A variety of sample target formats, including standard microtiter plate format 96 or 384 well targets, can be used. In addition, Fleximass™ microscope slides (plain or 48 well targets) and a wide variety of adaptors for unconventional sample layouts are available.
Like all AXIMA MALDI-TOF systems, the AXIMA Assurance can be fully integrated with robotic sample preparation devices, including the AccuSpot MALDI plate spotter and CHIP chemical printer, to create application-specific platforms. Integration with the CHIP creates a powerful platform for tissue imaging, while using an AXIMA instrument with a Prominence GPC system and AccuSpot results in a fully automated platform for polymer analysis that delivers quick, detailed analysis of ultra-trace substances. ... Read More | The OmegaTOF system couples Shimadzu’s benchtop MALDI-TOF instrument platform (MALDI-8020) with established technology for high mass detection from CovalX Instruments Inc. The result is a user-friendly, compact platform that extends benchtop MALDI-TOF analysis to a mass range of 1.5 MegaDalton.
TheThe OmegaTOF system couples Shimadzu’s benchtop MALDI-TOF instrument platform (MALDI-8020) with established technology for high mass detection from CovalX Instruments Inc. The result is a user-friendly, compact platform that extends benchtop MALDI-TOF analysis to a mass range of 1.5 MegaDalton.
The high mass detection capabilities of the OmegaTOF allow the highest sensitivity for analyzing large biomolecules, protein interactions, peptides, oligonucleotides and polymers. This allows access to a full range of applications already developed and optimized by CovalX technologies, most notably characterizing monoclonal antibodies, such as IgG, IgA and IgM. Crosslinking chemistries developed by CovalX enable the OmegaTOF to measure intact protein interactions directly, providing insight into protein aggregation, mAb:antigen interactions, protein complex stoichiometry or ligand association/dissociation measurements.
The OmegaTOF offers: - 200 Hz solid-state 355 nm laser featuring a class-leading two billion shots per lifetime
- Fast sample introduction using a load-lock chamber and quick sample stage movement
- Integrated barcode reader
- Patented TrueClean™ source cleaning
WideBore™ ion optics technologies that significantly reduce contamination from desorbed MALDI samples - Ease-of-use
- Low maintenance
- Small footprint
- Quiet operation at <55 dB
OmegaTOF data can be directly imported into the latest versions of CovalX ComplexTracker software for protein interaction analysis. Tailored MALDI Solutions acquisition software features data security for regulatory compliance facilitated with password-controlled user login, encrypted data, and user traceability. ... Read More |
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