Capillary electrophoresis instruments enable to the separation of charged molecules from each other. Unlike standard SDS-PAGE separation, capillary electrophoresis systems can provide molecule detection after separation through a light source and detector and the system can utilize high voltages without overheating the samples. Applications for capillary electrophoresis instruments include pharmaceutical (i.e. digest analysis of biopharma drugs; peptide mapping), life science (i.e., protein/peptide separation, DNA analysis, and PCR product analysis, chemical (i.e. raw material analysis), forensics (i.e. drug and explosive analysis), environmental (i.e. pesticide analysis) and food/beverage (i.e. as organic acid analysis).
Consideration when purchasing a capillary electrophoresis system:
Capillary electrophoresis provides additional advantages over other analysis methods, like HPLC, GC, SDS-PAGE, which include speed, cost of analysis, and easy method development. First consideration is the type of system required. There are several methods of capillary electroseparation, which include capillary zone electrophoresis, capillary isoelectric focusing, capillary gel electrophoresis, and more. Second consideration is the system’s throughput; how many samples can the instrument run.
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| Company | Agilent Technologies | Agilent Technologies | Agilent Technologies | Agilent Technologies | Agilent Technologies |
| Item | Femto Pulse System | Fragment Analyzer Systems | Oligo Pro II System | ProteoAnalyzer System | ZAG DNA Analyzer System |
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| Catalog Number | M5330AA | M5310AA / M5311AA / M5312AA | M5340AA | M5350AA | M5320AA |
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| Description | By using a pulsed-field power supply, large molecular weight DNA fragments can be separated and nucleic acids detected into the femtogram range. Compared to other non-denaturing gel-based analytical instruments, the Femto Pulse system easily achieves 10 times higher sensitivity for nucleic acid By using a pulsed-field power supply, large molecular weight DNA fragments can be separated and nucleic acids detected into the femtogram range. Compared to other non-denaturing gel-based analytical instruments, the Femto Pulse system easily achieves 10 times higher sensitivity for nucleic acid smears and up to 100 times higher sensitivity for nucleic acid fragments.
The ability to separate high molecular weight DNA up to 165 kb and detect nucleic acids down to 50 fg/µL input concentration makes the Femto Pulse system ideal for long-read NGS QC, gDNA, small RNA, or cfDNA analysis from low-concentration samples.
Features - Large Fragment Resolution - Separate high molecular weight DNA smears and fragments through 165 kb in about 1.5 hours.
- Femtogram Level Sensitivity - Achieve 10 times higher sensitivity for nucleic acid smears and up to 100 times higher for nucleic acid fragments.
- Simple Sample Preparation - Minimize sample degradation with fewer pipetting steps and stable reagents.
- Powerful Analysis Software - One intuitive software program automates the analysis of diverse sample types.
- Conserve Precious Sample - Unparalleled sensitivity allows you to use less precious samples and achieve superior detection.
- Make Workflows More Efficient - Eliminate pulsed-field gel electrophoresis from long-read NGS library preparation and BAC analysis.
... Read More | The Agilent Fragment Analyzer systems employ several unique design features to alleviate common quality control chokepoints. These features include increased instrument availability, flexible and dependable operations, and customizable runs helping you reach results faster.
Using automated parallelThe Agilent Fragment Analyzer systems employ several unique design features to alleviate common quality control chokepoints. These features include increased instrument availability, flexible and dependable operations, and customizable runs helping you reach results faster.
Using automated parallel capillary electrophoresis, the Fragment Analyzer systems offer high-resolution nucleic acid quality control for a range of applications, including NGS libraries, genomic DNA, cfDNA, total RNA, and IVT mRNA.
Features:
- Low to ultra-high throughput separation
- Analyze 12, 48, or 96 samples per run using parallel capillary electrophoresis
- Optimized for speed or resolution
- Choose from different capillary array lengths for either fast or high-resolution analysis
- High-resolution separation
Achieve separation resolution as good as 3 bp from fragments under 300 bp
- Seamless switching between applications
Load two different gels into the instrument to use different kits within one system setup
... Read More | The Agilent Oligo Pro II system delivers accurate purity assessments of ssDNA and ssRNA oligonucleotides without the use of dyes. Ensure optimum downstream performance of oligonucleotides up to 60-mer in length with n-1 resolution.
With 12-, 24-, and 96-capillary arrays available along with The Agilent Oligo Pro II system delivers accurate purity assessments of ssDNA and ssRNA oligonucleotides without the use of dyes. Ensure optimum downstream performance of oligonucleotides up to 60-mer in length with n-1 resolution.
With 12-, 24-, and 96-capillary arrays available along with three 96-well plate sample drawers, the Oligo Pro II system quickly adjusts to meet changing throughput demands. Intuitive and automated operations simplify the measurement of oligonucleotide purity and help you focus on results.
Features - Fast separations - Complete 12, 24, or 96 samples in about 1 hour
- Improve product quality - Monitor synthesis performance to demonstrate purity of ssDNA or ssRNA
- Wide sizing range - Achieve single base resolution through 60-mers
- Throughput for all labs - Variable throughput with the option of 12, 24, or 96 capillary arrays
- Direct detection of oligos - No dye labeling needed, uses UV detection
- Simplified report generation - Advanced peak integration software and data reporting capabilities
... Read More | The Agilent ProteoAnalyzer system is a parallel capillary electrophoresis instrument that automates the capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) analysis of reduced and non-reduced protein samples. Able to separate 12 samples in as little as 30 minutes, this system is designed to The Agilent ProteoAnalyzer system is a parallel capillary electrophoresis instrument that automates the capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) analysis of reduced and non-reduced protein samples. Able to separate 12 samples in as little as 30 minutes, this system is designed to eliminate the laborious process of SDS-PAGE gel preparation, staining/destaining, and analysis so you can focus on results.
The ProteoAnalyzer system separates proteins from 10 to 240 kDa allowing for the analysis of proteins over a wide range of sizes in a single run. The discrete individual capillaries are rejuvenated between every separation allowing for the analysis of diverse samples including monoclonal antibodies, biosimilars, protein purification fractions, and crude lysates.
Features: - Short analysis time— Analyze 12 protein samples in parallel in as little as 30 minutes
- Various sample types— Consistently separate challenging protein samples such as membrane proteins and crude lysates
- High separation resolution— Resolve glycosylated and non-glycosylated isoforms
- Broad sizing range— Agilent Protein Broad Range P240 kit for sizing proteins from 10 to 240 kDa
- High sensitivity— Detect as low as 1 ng/µL concentration of BSA protein in PBS
- Wide sample concentration range— Linear dynamic range of 2 - 2,000 ng/µL BSA sample in PBS
- Improved lab efficiency— Load samples and program next runs while a separation is currently in process
- Flexible sample loading— Move samples up or down in queue to adjust run priorities
- Minimal instrument preparation— Does not require daily array handling and utilizes room-temperature stable reagents
... Read More | The Agilent ZAG DNA Analyzer system relieves bottlenecks in automated high-throughput DNA fragment analysis workflows. Using parallel capillary electrophoresis, the ZAG DNA Analyzer system can process thousands of DNA fragment samples a day, eliminating bottlenecks associated with agarose gels.
The Agilent ZAG DNA Analyzer system relieves bottlenecks in automated high-throughput DNA fragment analysis workflows. Using parallel capillary electrophoresis, the ZAG DNA Analyzer system can process thousands of DNA fragment samples a day, eliminating bottlenecks associated with agarose gels.
This system is ideal for various DNA fragment applications, from basic PCR fragment analysis to microsatellite detection. Minimal sample preparation, automated separation of samples, and the option for continuous operation help keep your workflow moving.
Features - High Separation Resolution - Reliably distinguish and size DNA fragments within 3 bp of each other.
- High Throughput - Process 48, 96-well sample trays in 24 hours.
- Broad Sizing Range - Size fragments between 35 bp and 20,000 bp through a variety of available DNA analysis kits.
- Fast Separation Times - Samples can be separated in as little as 20 minutes.
- Intuitive Data Analysis - Analytical software can process separation data automatically after the run and generate a report in multiple formats.
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| Type | Quantitative | Inquire | Inquire | Inquire | Qualitative |
| Applications | High molecular weight DNA, large DNA fragment libraries, BAC clones, low concentrated RNA and DNA sample QC, cfDNA | Next-Gen Sequencing libraries, PCR Fragments, Cell-Free DNA, Genomic DNA, total RNA, and IVT mRNA | Accurate purity assessments of ssDNA and ssRNA oligonucleotides without the use of dyes | Protein sizing and quantification under reduced and non-reduced conditions; antibodies analysis, membrane, secreted, purified protein, and total protein extractions analysis | Genotyping, restriction digest analysis, PCR amplicon analysis, microsatellite detection |
| Number of Samples | 12 (per run) | 12 to 96 samples | up to 96 | 12 per run | 96 samples in less than 20 minutes |
| Voltage | 100 to 240 VAC | 100 to 240 VAC | 100 to 240 VAC | 100 to 240 VAC | 100 to 240 VAC |
| Dimensions | (WxDxH) 36 x 51 x 61 cm | (WxDxH) 36 x 51 x 61 cm | (WxDxH) 49 x 58 x 67 cm | (WxDxH) 36 x 51 x 61 cm | (WxDxH) 61 x 59 x 67 cm |
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