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| Company | CYTENA | CYTENA | CYTENA | CYTENA | CYTENA |
| Item | B.SIGHT™ Microbial Single Cell Isolation | F.SIGHT™ 2.0 Next-Gen Single Cell Dispenser with Fluorescent Sorting | F.SIGHT™ OMICS Single Cell Dispenser for NGS Library Preparation Workflows | F.SIGHT™ Single Cell Dispenser with Fluorescent Sorting | UP.SIGHT™ Single Cell Dispenser and Plate Imager |
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| Catalog Number | B.SIGHT | CY.US.UCA.204 | F.SIGHT OMICS | F.SIGHT | CY.US.UCA.233 |
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| Description | The second generation of B.SIGHT is a microbial isolation platform that uses cutting-edge optical microscopy and our single-cell dispensing technology to enable the isolation of the smallest microbial cells. This device revolutionizes microbiology research by allowing rapid and efficient isolation The second generation of B.SIGHT is a microbial isolation platform that uses cutting-edge optical microscopy and our single-cell dispensing technology to enable the isolation of the smallest microbial cells. This device revolutionizes microbiology research by allowing rapid and efficient isolation of bacteria from complex microbiome samples or transformed yeast and/or bacteria cultures used for strain development.
Key features or benefits: - Fast high-throughput single- cell isolation and cloning of microorganism
- Isolate single bacteria/yeast in a 384-well plate in under 8 minutes
- Precise deposition of single cells onto PCR plates
- Assurance of clonality
- Can be integrated into automation workflows
... Read More | Rapid single-cell dispensing with fluorescent sorting.
The F.SIGHT™ 2.0 is our fastest single-cell dispenser capable of isolating both unlabeled and green fluorescent cells. With our patented, highly efficient, and gentle technology, single cells are dispensed into a 96-well plate in under 2 Rapid single-cell dispensing with fluorescent sorting.
The F.SIGHT™ 2.0 is our fastest single-cell dispenser capable of isolating both unlabeled and green fluorescent cells. With our patented, highly efficient, and gentle technology, single cells are dispensed into a 96-well plate in under 2 minutes, and into a 384-well plate in under 7 minutes. Its innovative dual-camera system enables you to simultaneously capture brightfield and fluorescence information at full resolution.
Key features and benefits: - Brightfield and fluorescence imaging
- Fast and gentle single cell dispensing
- Small footprint and benchtop
... Read More | The new microfluidic cell sorter that dispenses single cells into PCR plates.
The F.SIGHT™ OMICS is a novel, easy-to-use benchtop cell sorter that reliably dispenses single cells into PCR plates and facilitates downstream reaction miniaturization. Sorting is carried out by brightfield image The new microfluidic cell sorter that dispenses single cells into PCR plates.
The F.SIGHT™ OMICS is a novel, easy-to-use benchtop cell sorter that reliably dispenses single cells into PCR plates and facilitates downstream reaction miniaturization. Sorting is carried out by brightfield image analysis and fluorescence with a 488 nm laser. The F.SIGHT OMICS uses CYTENA's patented dispensing technology, which is compatible with sensitive cell types and minimizes cell stress. The Automatic Offset Correction allows reproducible, targeted delivery of individual cells to the center of each well.
The F.SIGHT OMICS needs only minimal startup time and does not require manual adjustments of sorting parameters. It can fill a 96-well plate in under 2 minutes and a 384-well plate in under 8 minutes. Additionally, the instrument’s simple, intuitive software interface helps capture a series of nozzle images for each cell that are available for additional analysis and verification of clonality.
Key features and benefits: - Sorting by bright-field image analysis and fluorescence
- Fast and gentle single cell dispensing
- Automated Offset Correction for precise dispensation into PCR plates
- Cell focusing technology
- Intuitive Software
... Read More | Fluorescent and label-free single-cell isolation for cell line development.
The F.SIGHT makes single-cell dispensing simpler and more efficient with our state-of-the-art fluorescent sorting. Design your individual fluorescence experiments within minutes with the option to use bright field imagingFluorescent and label-free single-cell isolation for cell line development.
The F.SIGHT makes single-cell dispensing simpler and more efficient with our state-of-the-art fluorescent sorting. Design your individual fluorescence experiments within minutes with the option to use bright field imaging for non-fluorescent cells. The F.SIGHT records and saves full-resolution bright field, fluorescence, and overlay images for future analysis and proof of clonality, giving you peace of mind that your results are accurate and comprehensive.
Key features and benefits: - Adjustable laser
- Detection of low-intensity dye or low copy numbers
- Laser for red fluorescence available (mCherry, Texas Red, RFP, Deep Red, etc.)
... Read More | The ultimate upgrade in cell line development.
The UP.SIGHT™ streamlines cell line development (CLD) workflows by automating labor-intensive and time-consuming steps. We have combined our patented, highly efficient, fast and gentle single-cell dispensing technology with a superfast, high-quality The ultimate upgrade in cell line development.
The UP.SIGHT™ streamlines cell line development (CLD) workflows by automating labor-intensive and time-consuming steps. We have combined our patented, highly efficient, fast and gentle single-cell dispensing technology with a superfast, high-quality imaging system that provides a full well image without image stitching. The UP.SIGHT offers two independent methods of assuring monoclonality: first, images are taken at the dispensing nozzle to ensure only one cell is placed in each well and nonclonal droplets are discarded; second, the single cells are confirmed once more with 3D Full Well Imaging, a new and innovative method that images the full volume of each well from the point of dispensing using the integrated imager, leading to a probability of clonality >99.99%.
Key features and benefits: - Double assured clonality
- Minimal cell loss
- Small footprint and benchtop
... Read More |
| Plate Formats | 96,384-well plates | 96- or 384-well plates | 96- and 384-well plates | 96- and 384-well plates | 96 or 384-well plates |
| Applications | Synthetic biology, Single bacteria isolation, Single yeast isolation, Microbiome research, Anaerobic microorganism isolation | Cell line development, Gene therapy and viral vector production, Cell therapy and stem cell-based research, CRISPR gene editing, Exosome manufacturing | Fluorescent single-cell isolation, Single-cell sorting for NGS library preparation, Single-cell proteomics, Single-cell transcriptomics, Single-cell multiomics | Cell line development, Gene therapy, Rare cell research and discovery, Monoclonal cell culture | Monoclonal cell culture, Monoclonal antibody development, Automated cell line development, Cell therapy & stem cell research, Gene Therapy |
| Volume | ~50 pL | ~200 pL | Inquire | ~200 pL | Inquire |
| Analysis Time | Single-cell dispensing 96-well plate: ~ 3 min Single-cell dispensing 384-well plate: ~ 8 min | Single-cell dispensing 96-well plate: ~ 2 min Single-cell dispensing 384-well plate: ~ 8 min | Inquire | Single-cell dispensing 96-well plate: ~ 5 min Single-cell dispensing 384-well plate: ~ 15 min | Single-cell dispensing into 96-well plate: ~2 min Single-cell dispensing into 384-well plate: ~8 min Single-cell dispensing plus 3D Full Well Imaging 384-well plate: ~25 min Well bottom imaging of 96-well plate: ~8 min Well bottom imaging of 384-well plate: ~8 min
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| Dimensions | (WxDxH) 635 x 400 x 282 mm | (WxDxH) 635 x 400 x 282 mm | (WxDxH) 635 x 400 x 282 mm | (WxDxH) 600 x 400 x 240 mm | (WxDxH) 635 x 400 x 282 mm |
| Cell size range | 0.5 to 20 µm | 5 to 40 µm | 5 to 40 µm | 5 to 40 µm | 5 to 40 µm |
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