Single cell dispensers are specialized laboratory instruments used to isolate and dispense single cells for various analytical techniques. Often utilizing a combination of microscopy and microfluidics techniques, single cell dispensers can isolate stained or unstained cells and gently dispense cells into various-sized well plates for analysis. Single cell dispensers are an indispensable instrument for streamlining cell line development (CLD) by utilizing automation while increasing reproducibility and efficiency while ensuring monoclonality. Single cell dispensers ensure monoclonality in several different ways and the methods used should be compared to ensure compliance with your specific assay. Plate formats should also be considered as these can vary from instrument to instrument and most instruments can accommodate multiple plate sizes. Plate sizes can also affect analysis time, with higher capacity plates leading to longer analysis times. Lastly, the size of your cells should be considered, single cell dispensers are often compatible with a range of cell sizes but compatibility with your specific cell sizes should be ensured.
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| Company | CYTENA | CYTENA | CYTENA |
| Item | F.SIGHT™ OMICS Single Cell Dispenser for NGS Library Preparation Workflows | F.SIGHT™ 2.0 Next-Gen Single Cell Dispenser with Fluorescent Sorting | F.SIGHT™ Single Cell Dispenser with Fluorescent Sorting |
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| Catalog Number | F.SIGHT OMICS | CY.US.UCA.204 | F.SIGHT |
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| Description | The new microfluidic cell sorter that dispenses single cells into PCR plates.
The F.SIGHT™ OMICS is a novel, easy-to-use benchtop cell sorter that reliably dispenses single cells into PCR plates and facilitates downstream reaction miniaturization. Sorting is carried out by brightfield image The new microfluidic cell sorter that dispenses single cells into PCR plates.
The F.SIGHT™ OMICS is a novel, easy-to-use benchtop cell sorter that reliably dispenses single cells into PCR plates and facilitates downstream reaction miniaturization. Sorting is carried out by brightfield image analysis and fluorescence with a 488 nm laser. The F.SIGHT OMICS uses CYTENA's patented dispensing technology, which is compatible with sensitive cell types and minimizes cell stress. The Automatic Offset Correction allows reproducible, targeted delivery of individual cells to the center of each well.
The F.SIGHT OMICS needs only minimal startup time and does not require manual adjustments of sorting parameters. It can fill a 96-well plate in under 2 minutes and a 384-well plate in under 8 minutes. Additionally, the instrument’s simple, intuitive software interface helps capture a series of nozzle images for each cell that are available for additional analysis and verification of clonality.
Key features and benefits: - Sorting by bright-field image analysis and fluorescence
- Fast and gentle single cell dispensing
- Automated Offset Correction for precise dispensation into PCR plates
- Cell focusing technology
- Intuitive Software
... Read More | Rapid single-cell dispensing with fluorescent sorting.
The F.SIGHT™ 2.0 is our fastest single-cell dispenser capable of isolating both unlabeled and green fluorescent cells. With our patented, highly efficient, and gentle technology, single cells are dispensed into a 96-well plate in under 2 Rapid single-cell dispensing with fluorescent sorting.
The F.SIGHT™ 2.0 is our fastest single-cell dispenser capable of isolating both unlabeled and green fluorescent cells. With our patented, highly efficient, and gentle technology, single cells are dispensed into a 96-well plate in under 2 minutes, and into a 384-well plate in under 7 minutes. Its innovative dual-camera system enables you to simultaneously capture brightfield and fluorescence information at full resolution.
Key features and benefits: - Brightfield and fluorescence imaging
- Fast and gentle single cell dispensing
- Small footprint and benchtop
... Read More | Fluorescent and label-free single-cell isolation for cell line development.
The F.SIGHT makes single-cell dispensing simpler and more efficient with our state-of-the-art fluorescent sorting. Design your individual fluorescence experiments within minutes with the option to use bright field imagingFluorescent and label-free single-cell isolation for cell line development.
The F.SIGHT makes single-cell dispensing simpler and more efficient with our state-of-the-art fluorescent sorting. Design your individual fluorescence experiments within minutes with the option to use bright field imaging for non-fluorescent cells. The F.SIGHT records and saves full-resolution bright field, fluorescence, and overlay images for future analysis and proof of clonality, giving you peace of mind that your results are accurate and comprehensive.
Key features and benefits: - Adjustable laser
- Detection of low-intensity dye or low copy numbers
- Laser for red fluorescence available (mCherry, Texas Red, RFP, Deep Red, etc.)
... Read More |
| Plate Formats | 96- and 384-well plates | 96- or 384-well plates | 96- and 384-well plates |
| Applications | Fluorescent single-cell isolation, Single-cell sorting for NGS library preparation, Single-cell proteomics, Single-cell transcriptomics, Single-cell multiomics | Cell line development, Gene therapy and viral vector production, Cell therapy and stem cell-based research, CRISPR gene editing, Exosome manufacturing | Cell line development, Gene therapy, Rare cell research and discovery, Monoclonal cell culture |
| Volume | Inquire | ~200 pL | ~200 pL |
| Analysis Time | Inquire | Single-cell dispensing 96-well plate: ~ 2 min
Single-cell dispensing 384-well plate: ~ 8 min | Single-cell dispensing 96-well plate: ~ 5 min
Single-cell dispensing 384-well plate: ~ 15 min |
| Dimensions | (WxDxH) 635 x 400 x 282 mm | (WxDxH) 635 x 400 x 282 mm | (WxDxH) 600 x 400 x 240 mm |
| Cell size range | 5 to 40 µm | 5 to 40 µm | 5 to 40 µm |
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