As their name implies, ultraviolet visible spectrophotometers (often referred to as a UV Vis spectrophotometer or just a UV spectrophotometer) quantify the optical properties of samples in the ultraviolet and visible wavelength ranges of light (typically 190 to 900/1100 nm). Specifically, UV visible spectroscopy determines how much light of a given wavelength passes through a sample, and how much is absorbed. Such information can be diagnostic of molecular identity, concentration, structure, or activity, whether of organic species such as porphyrins, benzenes, and napthalenes, or of biomolecules like nucleic acids and proteins. Spectrophotometers can scan either fixed (i.e., predefined) or variable wavelengths, and come in both single- and double-beam configurations.
Find, compare, and request a quote for UV VIS spectrophotometers across leading suppliers at Labcompare; including access to product citations, related published figures, and first-hand reviews from our scientific community.
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| Company | Agilent Technologies | Agilent Technologies | Agilent Technologies | Agilent Technologies |
| Item | Cary 4000 UV-Vis Spectrophotometer | Cary 5000 UV-Vis-NIR Spectrophotometer | Cary 60 UV-Vis Spectrophotometer | Cary 6000i UV-Vis-NIR Spectrophotometer |
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| Catalog Number | 10079200 | 10079300 | Cary 60 | 10079400 |
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| Optical System | Double Beam | Double Beam | Double beam | Double out-of-plane Littrow monochromator |
| Wavelength Range | 175 to 900 nm | 175 to 3300 nm | 190 to 1100 nm | 175 to 1800 nm |
| Resolution | Inquire | Inquire | Inquire | UV/Vis less than 0.05; NIR less than 0.1 |
| Scanning Speed | UV-Vis 2,000 nm/min | UV-Vis 2000 nm/min; NIR 8000 nm/min | 24,000 nm/min, 80 data points/sec | UV-Vis 2,000 nm/min; NIR 8,000 nm/min |
| Spectral Bandwidth | UV-Vis 0.01 - 5.00 nm | Down to 0.01 nm | 1.5 nm fixed | UV-Vis 0.01 - 5.00 nm; NIR 0.04 - 20 nm |
| Light Source | Tungsten halogen visible and deuterium arc UV | Tungsten halogen visible and deuterium arc UV | Xenon flash lamp | Mercury lamp |
| Description | The Cary 4000 is a high-end UV-Vis spectrometer with excellent photometric performance in the 175–900 nm range, making it a powerful reference spectrometer. The system is ideal for analyzing solid samples or biotechnology samples with minimal sample preparation.
Controlled by the Cary WinUV software,The Cary 4000 is a high-end UV-Vis spectrometer with excellent photometric performance in the 175–900 nm range, making it a powerful reference spectrometer. The system is ideal for analyzing solid samples or biotechnology samples with minimal sample preparation.
Controlled by the Cary WinUV software, a Windows-based modular software, it can be ordered with specific modules for bio and pharma.The UV-Vis spectrometer has a number of optional accessories for liquid samples, like temperature control and multicell holders.
Features
- Measure beyond 8.0 absorbance units with reference beam attenuation
- WinUV software—a variety to applications from scanning to kinetics and thermal
- Versatile suite of accessories including temperature control, liquid and solid sample holders, solids autosamplers, multicell holders, and diffuse and specular reflectance accessories
- Variable slit widths (down to 0.01 nm) for optimum control over data resolution
- Large sample compartment with precision LockDown mechanism for quick changes of accessories
- Maximum light throughput using Schwarzchild coupling optics
- Minimal noise and stray light using a floating aluminum casting and double out-of-plane Littrow monochromator
- Extended dynamic range by attenuating the reference beam to be more equivalent to the samples absorbance
- Nitrogen pumping—separate purging in the sample and monochromator compartments
... Read More | The Cary 5000 is a high-performance UV-Vis and NIR spectrophotometer with superb photometric performance in the 175–3300 nm range. Using a PbSmart detector, the Cary 5000 extends its NIR range to 3300 nm making it a powerful tool for materials science research. The Cary WinUV software, a modular The Cary 5000 is a high-performance UV-Vis and NIR spectrophotometer with superb photometric performance in the 175–3300 nm range. Using a PbSmart detector, the Cary 5000 extends its NIR range to 3300 nm making it a powerful tool for materials science research. The Cary WinUV software, a modular Windows-based software, makes it easy to perform powerful analysis and control a number of optional accessories.
The large sample compartment can be expanded to hold large accessories and integrating spheres for spectral and diffuse reflectance. The LockDown mechanism makes it possible to quickly change and position accessories for reproducible results.
Features - Measure beyond 8.0 absorbance units with reference beam attenuation
- Measure 175 to 3300 nm using a PbSmart NIR detector for extended photometric range
- WinUV software—modular software with power analysis and enhanced transfer and report export capabilities
- Versatile set of accessories for materials research including spectral and diffuse reflectance
- Variable slit widths (down to 0.01 nm) for optimum control over data resolution
- Large sample compartment with LockDown mechanism for quick changes and positioning for reproducible results
- Maximum light throughput using Schwarzchild coupling optics for higher accuracy at low transmission levels
- Minimal noise and stray light using a floating aluminum casting and double Littrow monochromator
- Extended dynamic range by attenuating the reference beam to balance double beam signal levels.
- Nitrogen purging—separate purging in the sample and monochromator compartments to reduce oxygen and water vapor in the ultra-violet.
... Read More | The Cary 60 UV-Vis spectrophotometer has a wavelength range of 190–1100 nm that can be scanned in under three seconds. Our flexible UV-Vis system can be fitted with long pathlength cuvettes and solid sample transmission or reflectance accessories, and is ideal for remote UV-Vis absorbance analysis, The Cary 60 UV-Vis spectrophotometer has a wavelength range of 190–1100 nm that can be scanned in under three seconds. Our flexible UV-Vis system can be fitted with long pathlength cuvettes and solid sample transmission or reflectance accessories, and is ideal for remote UV-Vis absorbance analysis, when fitted with fiber optic probes.
The xenon source lamp has a 10 year replacement warranty (for Cary 60 instruments purchased from Agilent or participating partners), requires zero warmup, and causes no photodegradation of samples. Fast reactions can be recorded with 80 data points per second. The Cary 60 is well-suited as a routine UV-Vis spectrophotometer or for use in teaching labs. Optional 21 CFR Part 11 software tools are available.
Features - Reduce time-consuming and expensive lamp replacements, and minimize instrument revalidation costs with the unique Agilent xenon source lamp.
- Safely measure life-science samples without sample photodegradation and ensure you get the correct answer every time with the Cary 60 UV-Vis spectrophotometer.
- Get reliable UV-Vis spectrophotometer performance with fiber-optic measurement probes. Why use cuvettes when you can bring the UV-Vis instrument to the sample and get accurate results in a fraction of the time?
- Measure small volumes and precious samples accurately and reproducibly with a highly focused beam image.
- Scan the entire wavelength range (190 to 1100 nm) in under three seconds and collect data from single or multiple wavelengths at 80 data points per second.
- Take advantage of the Cary 60 UV-Vis spectrophotometer’s comprehensive range of accessories to accurately characterize the widest variety of liquid and solid samples.
- Tailor the Cary WinUV software to suit your analytical requirements. From classroom to research laboratories through R&D and regulated QA/QC environments, simplify your UV-Vis spectroscopy measurements and finish more in less time.
- Choose sustainability: The Cary 60 UV-Vis received My Green Lab’s ACT (Accountability, Consistency, Transparency) label after an independent audit for its environmental impact throughout the product lifecycle.
... Read More | The Cary 6000i UV-Vis-NIR spectrophotometer has been tailored for unmatched photometric performance in the short-wave infrared (SWIR) 800–1800 nm range as well as the UV-visible 175 to 800 nm. Using narrow-band InGaAs detection and a 600 lines-per-mm diffaction grating for improved SWIR sensivity, The Cary 6000i UV-Vis-NIR spectrophotometer has been tailored for unmatched photometric performance in the short-wave infrared (SWIR) 800–1800 nm range as well as the UV-visible 175 to 800 nm. Using narrow-band InGaAs detection and a 600 lines-per-mm diffaction grating for improved SWIR sensivity, the Cary 6000i is the ultimate tool for materials characterization spectroscopy.
Using Cary WinUV software with the Cary 6000i makes it easy to perform powerful analysis and control optional accessories. The large sample compartment can be expanded to hold large accessories and integrating spheres for spectral and diffuse reflectance. The LockDown mechanism makes it possible to quickly change and position accessories for reproducible results.
Features
- Measure beyond 8.0 absorbance units with reference beam attenuation
- Measure 175 to 1800 nm using a narrow-band InGaAs detector and 600 l/mm NIR diffraction gratings for unmatched sensivity in the short-wave infrared
- WinUV software—modular software with power analysis and enhanced transfer and report export capabilities
- Versatile set of accessories for materials research including spectral and diffuse reflectance
- Variable slit widths (down to 0.01 nm) for optimum control over data resolution
- Large sample compartment with LockDown mechanism for quick changes and positioning for reproducible results
- Maximum light throughput using Schwarzchild coupling optics for higher accuracy at low transmission levels
- Minimal noise and stray light using a floating aluminum casting and double out-of-plane Littrow monochromator
- Extended dynamic range by attenuating the reference beam to balance double beam signal levels.
- Nitrogen purging—separate purging in the sample and monochromator compartments to reduce oxygen and water vapor in the ultra-violet.
... Read More |
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