UV VIS Spectrophotometer

The Shimadzu BioSpec-nano is a low-maintenance micro-volume spectrophotometer designed for the modern life science laboratory. It offers superior detection limits, up to 10 times better compared to the competition, making it the perfect instrument for quantitation of DNA, RNA, protein analysis, and

The Shimadzu BioSpec-nano is a low-maintenance micro-volume spectrophotometer designed for the modern life science laboratory. It offers superior detection limits, up to 10 times better compared to the competition, making it the perfect instrument for quantitation of DNA, RNA, protein analysis, and photometric measurements. Quantitation can be rapidly and simply carried out with very small samples ranging from 1µL. And, with dedicated functions, such as automated sample mounting and wiping, the BioSpec-nano offers a rapid 3-second analysis time and a 10-second cycle time between samples.

Features

Ultra-small Sample Volumes
Perform analysis with 1µL to 2µL samples. For ultra-small sample volumes no standard rectangular cell is needed although for applications that are not volume-limited, an optional rectangular cell adapter is available.

Automated Analysis
With “drop-and-click” functionality, analysis has never been easier. Simply drop the sample onto the target and click the button.

Easy Operation/Maintenance
Sample mounting, measurement and cleaning are conducted by the instrument, eliminating the need for operators to perform the tedious, repetitive, and inconsistent placement of the fiber optic element and manual cleaning required in other instruments.

Speed
Measurement takes seconds, and a series of samples can be analyzed while confirming spectra in the Detail view mode.

Specifications
Measuring wavelength range: 220 to 800 nm
Spectrum band width: 3 nm
Wavelength accuracy: ± 1 nm
Pathlength: 0.2 mm, 0.7 mm (manual selection)
Light source: Xenon flash lamp
Monochrometer: Holographic grating
Detector: Photo diode array
Auto wiping function: Provided
Sample mount function: Auto
Quantitation range: (OD, dsDNA concentration)
Pathlength 0.2 mm, 1 to 75 OD (50 to 3,700 ng / µL)
Pathlength 0.7 mm, 0.3 to 21 OD (15 to 1,000 ng / µL)
Optional 5 mm pathlength cell, 0.04 to 3 OD (2 to 150 ng / µL)

• Photometric mode allows users to measure the absorbance or transmittance at single or multiple wavelengths. It is also capable of quantitation using the k-factor method.
• Spectrum mode records a sample spectrum using wavelength scanning. Users can repeat scans to follow sample changes over time.
• Quantitation mode generates a calibration curve from the measurement of standards and then calculates the concentrations of unknowns. Multi-component quantitation mode quantitates up to eight components mixed in a single sample.
• Kinetics mode measures absorbance changes as a function of time and obtains the enzymatic activity values.
• Time scan mode measures the change in absorbance, transmittance or energy as a function of time.
• Biomethod mode is used to determine DNA and protein concentrations using several quantitation methods, including the Lowry, BCA, Biuret, CBB and UV absorption methods.