In Raman microscopy, also called confocal Raman microscopy, an optical microscope and a Raman spectrometer are combined into one instrument in order to provide high resolution images of small samples. This high resolution imaging has applications that include materials science, earth science, cell biology, medicine, cosmetics, and art analysis.
A Raman microscope consists of a basic optical microscope, a Raman spectrometer, a laser, a light source, and a detector such as a CCD camera. The optical microscope uses light to magnify and identify samples while the Raman spectrometer scatters light and measures the excitation vibration. When selecting a microscope, it is important to consider the qualities of each of the microscope's components and their optimal configuration for a particular application.
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| Company | Bruker Optics | Bruker Optics |
| Item | SENTERRA II Dispersive Raman Microscope | RamanScopeIII FT-Raman Microscope |
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| Catalog Number | BOPT SENTERRA | BOPT RamanScopeIII |
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| Description | The SENTERRA is a high performance Raman microscope spectrometer designed for the most demanding analytical and research applications. Its most important innovation is certainly its internal continuous calibration as it ensures highest wavenumber accuracy without the need for calibrations with The SENTERRA is a high performance Raman microscope spectrometer designed for the most demanding analytical and research applications. Its most important innovation is certainly its internal continuous calibration as it ensures highest wavenumber accuracy without the need for calibrations with external standards.
Features - Continuous calibration with Sure_Cal®
- Compact design with spectrometer included in the microscope
- Multiple wavelengths: 1064nm, 830nm, 785nm, 633nm, 532nm and 488nm
- High performance confocal depth profiling with FlexFocusTM
- Automatic Fluorescence Rejection (AFR) using SERDS
- Open architecture version for the study of larger samples (e.g. in art) with high lateral resolution
- Inverted version for the investigation of living cells
- Coupling with Atomic Force Microscopy (AFM)
Compact and Rugged Design
Most commercial Raman microscopes employ spectrometers that are separate from the microscope. Therefore, alignment and maintenance of these devices is time consuming. SENTERRA integrates a multi-laser Raman spectrometer between the reflected light illuminator and the binocular of the microscope. SENTERRA’s compact design provides a short beam path, which increases sensitivity and makes it more robust and stable.... Read More | Today, with the culmination of more than 20 years experience, Bruker Optics offers state-of-the-art infrared and Raman microscopes. RamanScopeIII is based on a next generation, 'hybrid' platform that can accommodate multiple wavelengths of Raman excitation. The new RamanScopeIII system can be Today, with the culmination of more than 20 years experience, Bruker Optics offers state-of-the-art infrared and Raman microscopes. RamanScopeIII is based on a next generation, 'hybrid' platform that can accommodate multiple wavelengths of Raman excitation. The new RamanScopeIII system can be coupled to Bruker Optics' multi-range fully digital FT-Raman module, the RAM II, as well as the standalone MultiRAM FT-Raman spectrometer.
Optical Microscopy
The RamanScopeIII is based on the Olympus BX series optical microscope, all the necessary tools for sample visualization and contrast enhancements such as the Koehler brightfield and darkfield illumination, polarized light, Nomarski differential interference contrast (DIC) and fluorescence are available.
Minimize Fluorescence Interference
When sample fluorescence is a problem, FT-Raman microanalysis with near infrared excitation (1064 nm) is frequently the only solution. As sample fluorescence can be orders of magnitude more intense than Raman scattering, the presence of fluorescence often precludes observation of Raman scattering. Excitation at 1064 nm is low enough in excitation energy such that fluorescence is rarely generated.
Sensitivity and Stability
High throughput optics and Bruker Optics’ unique liquid nitrogen cooled Germanium detector offers ultra-low signal detection with minimal noise assuring excellent sensitivity. The long hold time of the refrigerant provides hassle free operation for an entire week. The inherent precision of the Bruker Optics FT-Raman instruments provides reliable long term stability, which is especially important for sample analyses requiring long acquisition times.
Combined Fourier Transform and Dispersive Raman Spectroscopy
The base system offers the long wavelength benefits of the fluorescence-free, non-destructive 1064 nm Fourier transform Raman excitation. The RamanScopeIII sits on an optical microscope, which can also accommodate the SENTERRA™ grating based dispersive Raman spectrometer. This combination provides full spectroscopic characterization and optimizes the strengths of the both techniques for your complex micro-analysis samples. By utilizing the multiple wavelengths, from 1064 nm to 532 nm on a single microscopic spot, the RamanScopeIII delivers excellent insight into many demanding applications, including forensics, pharmaceutical and polymer science.
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| Measurement Mode(s) | Confocal Raman, Automatic Fluorescence | FT-Raman spectrometer |
| Focal Length | Inquire | Inquire |
| Detector(s) | Grating | InGaAs detector or Ge diode detector |
| Laser Sources | Multiple laser sources | Diode-pumped, air-cooled Nd:YAG laser source |
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