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| Company | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology |
| Item | miniDAWN™ Multi-Angle Light Scattering (MALS) Detector for SEC-MALS | DAWN™ Multi-angle Light Scattering (MALS) Instrument | DynaPro™ Plate Reader III Dynamic Light Scattering Instrument | DynaPro™ ZetaStar™ Dynamic, Electrophoretic, and Static Light Scattering Instrument | WyattQELS™ Light Scattering Detector Module |
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| Catalog Number | miniDAWN | DAWN | DynaPro Plate reader III | DynaPro ZetaStar | WyattQELS |
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| Description | Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected sample, 100 kDa polystyrene in THF (varies for other samples and solvents). The miniDAWN is engineered with self-contained modules that are easily replaced making it field serviceable. The system-ready monitors soise levels in real-time and an all-green status indicator lets you know when your system is ready for data collection. The optional DLS module uses the WyattQELS™ module, which fits inside the miniDAWN to providing on-line dynamic light scattering for determining radii down to 0.5 nm. All light scattering cells are subject to contamination by particles and to combat this issue the instrument has a self-cleaning, embedded COMET™ ultrasonic module. For maximum reliability and uptime, program the miniDAWN to automatically clean the cell after every run. The units may also be combined with a Calypso™ composition-gradient system to characterize biomolecular interactions.... Read More | The DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar massThe DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass, size, conformation, particle concentration, conjugation (e.g. glycoproteins) or encapsulation (e.g. AAV capsid content). It stands out with exceptional sensitivity and the widest range of molecular weight, size, and concentration of any MALS instrument, paired with a broad selection of configurations and optional modules for enhanced capabilities.
The DAWN is most often used in conjunction with gel permeation chromatography (GPC-MALS), size exclusion chromatography (SEC-MALS) or an EclipseTM, field-flow fractionation instrument (FFF-MALS) and may also be used with ion-exchange chromatography or reverse-phase chromatography. The instrument includes a high-resolution, touch-screen front panel to display data graphs, system health and instrument diagnostics and is accompanied by ASTRA™, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.... Read More | The DynaPro™ Plate Reader III is a high-throughput DLS instrument that delivers rapid measurements of particle size, concentration, aggregation, and stability of many different macromolecular or nanoparticle samples. It enables fully-automated screening of processing or formulation conditions The DynaPro™ Plate Reader III is a high-throughput DLS instrument that delivers rapid measurements of particle size, concentration, aggregation, and stability of many different macromolecular or nanoparticle samples. It enables fully-automated screening of processing or formulation conditions - simply load and walk away. Measurements are completed in standard 96, 384 or 1536 disposable well plates and the instrument can be integrated with plate and liquid-handling robots for additional time savings.... Read More | Unique among its peers, the DynaPro™ Zetastar™ performs simultaneous dynamic and static light scattering or dynamic and electrophoretic light scattering (DLS/SLS/ELS) measurements all within one instrument. Measure manually with microcuvettes, or automatically via an autosampler and flowUnique among its peers, the DynaPro™ Zetastar™ performs simultaneous dynamic and static light scattering or dynamic and electrophoretic light scattering (DLS/SLS/ELS) measurements all within one instrument. Measure manually with microcuvettes, or automatically via an autosampler and flow cell. Quickly quantify size, polydispersity, zeta potential/charge, particle concentration, molar mass or turbidity for characterization and stability studies.
Key Features:
- DYNAMICS™ Touch on-board app for walk-up, self-guided operation
- Adaptive collection for faster and simpler measurements of zeta potential
- Measure size and turbidity with just 2 µL of solution, or zeta potential with as little as 65 µL of solution
- Measure zeta potential in buffers with conductivity up to 2x physiological saline
- A selection of high-quality quartz and disposable cells and cuvettes for both size and zeta potential measurements
... Read More | The WyattQELS™ dynamic light scattering (DLS) module integrates into a DAWN™, miniDAWN™ or microDAWN™ online multi-angle light scattering (MALS) instrument in order to determine high-resolution particle size distributions, when used in conjunction with a separation technique The WyattQELS™ dynamic light scattering (DLS) module integrates into a DAWN™, miniDAWN™ or microDAWN™ online multi-angle light scattering (MALS) instrument in order to determine high-resolution particle size distributions, when used in conjunction with a separation technique such as size exclusion chromatography (SEC) or Eclipse™, field-flow fractionation (FFF). The measurement is performed at exactly the same time and in the same sample volume as MALS so Rh and Rg can be compared for particle conformation insights.
Applications include the characterization of the conformation of polymers and biomolecules, detection of protein unfolding, and identification of nanoparticles and vesicles based on shape and structure. One can also use QELS insights to determine optimal chromatography conditions based on molecular conformation.... Read More |
| Type | MALS and optional DLS | MALS and optional DLS | Dynamic Light Scattering / Static Light Scattering | Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS) | Dynamic Light Scattering |
| Particle Size | Inquire | Inquire | 0.5 to 1000 nm | 0.2 to 1000 nm | Flow mode: 1 nm to 40 nm (TREOS); 1 nm to 300 (HELEOS)
Batch mode: 10 to 50 nm; 0.5 nm to 1 µm (cut 10 to 50 nm) |
| Detector(s) | High-gain, high-dynamic range photodiodes at 3 detection angles for MALS. Optional avalanche photodiode for simultaneous dynamic light scattering measurements. | High-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS.
Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength. | Linearized avalanche photodiode for dynamic and static light scattering | Inquire | Avalanche photodiode coupled to a multimode optical fiber |
| Measuring Time | Depedent on LC separation | Depedent on LC separation | Measurement Time: 5 to 60 seconds per well
830 nm | 15 seconds typical | Measurement Time: 0.1 to 3600 sec
Same as MALS host instrument |
| Light Source | 658 nm laser, programmable from 10% to 100% maximum power. | 658 nm laser, programmable 10% - 100% of maximum power.
Optional: 785 nm for fluorescence suppression | 830 nm, fiber-coupled diode laser | 785 nm laser, programmable 10% to 100% | Uses the host MALS instrument's laser, usually a 658 nm laser. |
| Applications | With SEC/GPC: determination of distributions of absolute molar mass, size, conformation, conjugate ratio. With Calypso CG-MALS: characterization of biomolecular interactions. | With standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio and particle concentration.
With Calypso composition-gradient system: label-free characterization of biomolecular interactions including self- and hetero-association as well as non-specific interactions. | Develop stable biopharmaceuticals, assess viral vector titer and aggregation, optimize protein buffers for crystallization, SAXS or chromatography, formulate theranostic nanoparticles, discover small-molecule and peptide inhibitors of protein-protein interactions | Analysis of proteins, gene vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, nano-emulsions, lipid and metallic nanoparticles, and more. | High-resolution size distributions with fractionation by SEC or FFF; conformation when combined with MALS molar mass and/or size data |
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