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| Company | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology |
| Item | DAWN™ Multi-angle Light Scattering (MALS) Instrument | DynaPro™ NanoStar™ II Dynamic Light Scattering Instrument | DynaPro™ ZetaStar™ Dynamic, Electrophoretic, and Static Light Scattering Instrument | microDAWN™ Multi-Angle Light Scattering Instrument for UHP-SEC-MALS | miniDAWN™ Multi-Angle Light Scattering (MALS) Detector for SEC-MALS |
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| Catalog Number | DAWN | DynaPro NanoStar II | DynaPro ZetaStar | microDAWN | miniDAWN |
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| Description | The DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar massThe DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass, size, conformation, particle concentration, conjugation (e.g. glycoproteins) or encapsulation (e.g. AAV capsid content). It stands out with exceptional sensitivity and the widest range of molecular weight, size, and concentration of any MALS instrument, paired with a broad selection of configurations and optional modules for enhanced capabilities.
The DAWN is most often used in conjunction with gel permeation chromatography (GPC-MALS), size exclusion chromatography (SEC-MALS) or an EclipseTM, field-flow fractionation instrument (FFF-MALS) and may also be used with ion-exchange chromatography or reverse-phase chromatography. The instrument includes a high-resolution, touch-screen front panel to display data graphs, system health and instrument diagnostics and is accompanied by ASTRA™, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.... Read More | The DynaPro™ NanoStar™ II is the only DLS instrument to provide a fully integrated, walk-up measurement experience so even novices can immediately measure particle concentration, characterize size, aggregation, quality solubility, and stability of proteins, viruses, or nanoparticles in The DynaPro™ NanoStar™ II is the only DLS instrument to provide a fully integrated, walk-up measurement experience so even novices can immediately measure particle concentration, characterize size, aggregation, quality solubility, and stability of proteins, viruses, or nanoparticles in solution.
Key Features:
- DYNAMICS™, touch on-board app for walk-up, self-guided operation
- Measure size and size distributions with just 2 µL of solution of solution
- Measure macromolecules molecular weight from 300 Da to 1 MDa
... Read More | Unique among its peers, the DynaPro™ Zetastar™ performs simultaneous dynamic and static light scattering or dynamic and electrophoretic light scattering (DLS/SLS/ELS) measurements all within one instrument. Measure manually with microcuvettes, or automatically via an autosampler and flowUnique among its peers, the DynaPro™ Zetastar™ performs simultaneous dynamic and static light scattering or dynamic and electrophoretic light scattering (DLS/SLS/ELS) measurements all within one instrument. Measure manually with microcuvettes, or automatically via an autosampler and flow cell. Quickly quantify size, polydispersity, zeta potential/charge, particle concentration, molar mass or turbidity for characterization and stability studies.
Key Features:
- DYNAMICS™ Touch on-board app for walk-up, self-guided operation
- Adaptive collection for faster and simpler measurements of zeta potential
- Measure size and turbidity with just 2 µL of solution, or zeta potential with as little as 65 µL of solution
- Measure zeta potential in buffers with conductivity up to 2x physiological saline
- A selection of high-quality quartz and disposable cells and cuvettes for both size and zeta potential measurements
... Read More | The microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size andThe microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects, the microDAWN is similar to the well-established miniDAWN, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius. The microDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion and produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC. In some cases it may be used with ion-exchange or other types of chromatography.... Read More | Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected sample, 100 kDa polystyrene in THF (varies for other samples and solvents). The miniDAWN is engineered with self-contained modules that are easily replaced making it field serviceable. The system-ready monitors soise levels in real-time and an all-green status indicator lets you know when your system is ready for data collection. The optional DLS module uses the WyattQELS™ module, which fits inside the miniDAWN to providing on-line dynamic light scattering for determining radii down to 0.5 nm. All light scattering cells are subject to contamination by particles and to combat this issue the instrument has a self-cleaning, embedded COMET™ ultrasonic module. For maximum reliability and uptime, program the miniDAWN to automatically clean the cell after every run. The units may also be combined with a Calypso™ composition-gradient system to characterize biomolecular interactions.... Read More |
| Type | MALS and optional DLS | Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) | Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS) | MALS and optional DLS | MALS and optional DLS |
| Particle Size | Inquire | 0.2 to 1000 nm | 0.2 to 1000 nm | Inquire | Inquire |
| Detector(s) | High-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS.
Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength. | Inquire | Inquire | High-gain, high dynamic range, static light scattering detectors at 3 angles | High-gain, high-dynamic range photodiodes at 3 detection angles for MALS. Optional avalanche photodiode for simultaneous dynamic light scattering measurements. |
| Measuring Time | Depedent on LC separation | Inquire | 15 seconds typical | Depedent on LC separation | Depedent on LC separation |
| Light Source | 658 nm laser, programmable 10% - 100% of maximum power.
Optional: 785 nm for fluorescence suppression | Inquire | 785 nm laser, programmable 10% to 100% | 660 nm laser, programmable 10% - 100% of maximum power. | 658 nm laser, programmable from 10% to 100% maximum power. |
| Applications | With standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio and particle concentration.
With Calypso composition-gradient system: label-free characterization of biomolecular interactions including self- and hetero-association as well as non-specific interactions. | Inquire | Analysis of proteins, gene vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, nano-emulsions, lipid and metallic nanoparticles, and more. | With SEC/GPC: determination of distributions of absolute molar mass, size, conformation, conjugate ratio. | With SEC/GPC: determination of distributions of absolute molar mass, size, conformation, conjugate ratio. With Calypso CG-MALS: characterization of biomolecular interactions. |
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