A fluorescence spectrophotometer (also referred to as a fluorometer, fluorospectrometer, or fluorescence spectrometer) is an instrument that measures the amount of fluorescent light that is emanated from a sample at different wavelengths post illumination by a xenon lamp. Fluorometers can have different settings for recording fluorescent signals which vary in their wavelengths. Some can also read fluorescent signals for fast, complex experiments, such as time-resolved fluorescent resonance energy transfer (FRET). Fluorometers can utilize cuvettes, capillaries, Petri dishes, or microplates to read samples. Fluorometers can also differ in the size of samples they process with some using only a very small amount of sample (down to 0.3 µl).
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| Company | JASCO | JASCO | JASCO | JASCO |
| Item | FP-8650 NIR Spectrofluorometer | FP-8250 Spectrofluorometer | FP-8350 Spectrofluorometer | FP-8550 Spectrofluorometer |
| Citations | | | | |
| Catalog Number | FP-8650 | FP-8250 | FP-8350 | FP-8550 |
| Price | | | | |
| Light Source | Continuous output Xe arc lamp with shielded lamp housing (150 W), Lifetime 3000 hours | Inquire | Continuous output Xe arc lamp with shielded lamp housing (150 W), Lifetime 3000 hours | Continuous output Xe arc lamp with shielded lamp housing (150 W), Lifetime 3000 hours |
| Wavelength Range | Ex: Zero order, 200 to 850 nm
Em: Zero order, 200 to 980 nm | Zero order, 200 to 750 nm | Zero order, 200 to 750 nm | Zero order, 200 to 850 nm |
| Wavelength Accuracy | Excitation: ± 1.0 nm (± 0.3 nm at 546.1 nm)
Emission: ± 2.0 nm (± 0.3 nm at 546.1 nm) | Excitation: ± 1.5 nm
Emission: ± 1.5 nm | Excitation: ± 1.5 nm
Emission: ± 1.5 nm | Excitation: ± 1.0 nm (± 0.3 nm at 546.1 nm)
Emission: ± 1.0 nm (± 0.3 nm at 546.1 nm) |
| Scanning Speed | Excitation: 10 to 60,000 nm/min
Emission: 20 to 120,000 nm/min | Excitation: 20 to 20,000 nm/min
Emission: 20 to 20,000 nm/min | Excitation: 20 to 20,000 nm/min
Emission: 20 to 20,000 nm/min | Excitation: 10 to 60,000 nm/min
Emission: 10 to 60,000 nm/min |
| Description | The FP-8650 Spectrofluorometer uses a uniquely red-sensitive PMT that extends the measurement range from the UV-Visible to the near infrared. Providing excitation wavelengths from 200 to 850 nm and emission detection up to 980 nm (1010 nm option). Samples such as carbon nanotubes, porphyrins and The FP-8650 Spectrofluorometer uses a uniquely red-sensitive PMT that extends the measurement range from the UV-Visible to the near infrared. Providing excitation wavelengths from 200 to 850 nm and emission detection up to 980 nm (1010 nm option). Samples such as carbon nanotubes, porphyrins and other NIR markers can easily be measured. It is especially well-suited for monitoring NIR-labeled biologicals far away from background auto-fluorescence The compact instrument design incorporates high-speed scanning and automatic cut-off filters to exclude higher-order diffraction for fast, accurate acquisition of single spectra and EEMs (Excitation-Emission-Matrices).
Features:
- High sensitivity S/N is 3500:1 (RMS)
- Many options include, solid sampling, spectral correction and quantum yield with ambient and LN2 cooled integrating spheres
- High-speed scanning up to 120,000 nm/min Emission
- Wavelength range: 200 to 1010 nm Emission (up to 850 nm, Excitation)
- Validation included
... Read More | The FP-8250 Spectrofluorometer is a user-friendly, general-purpose instrument that allows for measurement with a quick press of a start button on top of the instrument. The simplified, yet sensitive design includes everything that is required for routine fluorescence measurements in a quality-The FP-8250 Spectrofluorometer is a user-friendly, general-purpose instrument that allows for measurement with a quick press of a start button on top of the instrument. The simplified, yet sensitive design includes everything that is required for routine fluorescence measurements in a quality-control or teaching lab. The standard Auto-Sensitivity Control System (Auto-SCS) and Auto-Gain features enable measurement over a wide range of concentrations using a single calibration method. The Spectra Manager™ Suite of spectroscopy software offers full system control, with easy-to-use tools for data processing and analysis.
Features: - High sensitivity S/N > 4500 (RMS)
- Dynamic range up to six digits
- High speed scanning up to 20,000 nm/min
- Wavelength range: 200 to 750 nm
... Read More | The FP-8350 Spectrofluorometer is an extremely sensitive spectrofluorometer that can be used in the broadest range of applications: biological, environmental, teaching labs, and core facilities. Many features make this a simple-to-use instrument; automatic cut-off filters (included as standard) The FP-8350 Spectrofluorometer is an extremely sensitive spectrofluorometer that can be used in the broadest range of applications: biological, environmental, teaching labs, and core facilities. Many features make this a simple-to-use instrument; automatic cut-off filters (included as standard) eliminate peaks due to second-order scatter, giving more confidence in artifact-free spectral measurement. Auto-Gain and Auto-SCS optimize the S/N for samples with large differences in concentration and fluorescence intensity, offering a wide dynamic range. This reduces the need for manual intervention of adjusting spectral band and sensitivity settings for on-scale measurement.
Single and multi-cell Peltier accessories provide exceptional temperature control for thermal studies such as molecular conformation and folding.
Automated broad wavelength polarizers can be used for a range of experiments including anisotropy for investigation of binding events. Rapid-kinetics and titration measurements can be automated with fully integrated stopped-flow and auto-titrator units. Solid samples can be measured with dedicated holders for powders, films, etc.
Features: - High sensitivity S/N 8000:1 typical
- High resolution with SBW to 1.0 nm
- Wavelength range: 200 to 750 nm (900 nm optional)
... Read More | The FP-8550 Spectrofluorometer has been designed for demanding research applications and has the same functionality and advantages of the FP-8350, but also includes an optimized optical design for very low stray-light and enhanced spectral purity. Combined with the most thorough spectral correction,The FP-8550 Spectrofluorometer has been designed for demanding research applications and has the same functionality and advantages of the FP-8350, but also includes an optimized optical design for very low stray-light and enhanced spectral purity. Combined with the most thorough spectral correction, material engineers and researchers are assured of accurate measurements for the evaluation of advanced materials. The FP-8550 performs with the highest sensitivity for fast measurement of samples with low level fluorescence, whether they are challenging biochemical systems or low quantum efficiency materials. High-speed scanning of phosphorescent samples and 3D spectra enable fast acquisition of high-quality, high-density data.
The FP-8550 optical bench has been developed to take advantage of the wide range of sampling accessories and applications, such as temperature-dependent melting and kinetics, anisotropy, FRET, spectral correction, and quantum yields.
Features: - High intensity continuous output Xenon arc lamp
- Energy monitoring of the excitation energy for stability
- Shutter to protect samples until measurement
- Automatic Stray light rejection filters
- High sensitivity S/N 8500:1 (RMS, water Raman)
- High-speed scanning up to 60,000 nm/min
- Wavelength range: 200 to 850 nm
- Validation accessory (standard)
... Read More |
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