The Monarch Mag PCR & DNA Cleanup Kit (5 μg) enables rapid and reliable purification and concentration of up to 5 μg of DNA, including both single- and double- stranded forms, from common enzymatic reactions such as PCR, restriction digestion, ligation, reverse transcription, and rolling circle amplification (RCA). Using a streamlined bind-wash-elute workflow with silica-coated magnetic beads, the kit delivers highly pure, inhibitor-free DNA ready for immediate use in downstream applications such as in vitro transcription (IVT), sequencing, restriction digestion, library construction, labeling, and other enzymatic manipulations. The magnetic bead format offers flexibility and scalability, supporting both manual and automated workflows. Additional modified protocols support varying DNA input amounts and sample volumes and enable purification of oligos, genomic DNA, and RCA products.
The kit includes magnetic beads, optimized buffers, and reagents, all compactly packaged. The magnetic bead technology in this kit eliminates the need for centrifugation or vacuum-based processing and requires no pH monitoring, with the optimized buffer chemistry.
Monarch kits are manufactured to ensure high quality and consistency. As with all Monarch kits, this product is designed with sustainability in mind, reducing plastic usage where possible and in the design of buffer bottles and other plastic components, while maintaining performance and reliability across a wide range of applications.