Saurat Dione

Saurat Dione

Engineer

Articles by Saurat Dione

  • PVDF Transfer Membranes

    Thursday, December 21, 2023
    I have been using this Immobilon-P Transfer membrane for all my western blot and it works very well. This membrane needs to be activated with ethanol and MilliQ (1:1) for 5 minutes before use. Moreover, the Immobilon-P Transfer membrane (0.45 µm) is mainly used for proteins greater than 20 kDa and ... read more
  • Eppendorf Refrigerated Microcentrifuge

    Thursday, December 21, 2023
    This microcentrifuge has many qualities including the fact that it is small and compact and that we can put up to 24 eppendorfs in it. Moreover, it quickly drops in temperature and can reach up to 13000 RCF. However, the main criticism would be the fact that we can only fit 1.5 and 2 mL eppendorfs ... read more
  • Eppendorf Multi-Dispenser Pipette

    Thursday, December 21, 2023
    I have been using this multi-pipette daily for the past few months to load my microplate for PCR. This dispenser is very easy to use and ensures that the exact same volume will be added to each well. Moreover, it is possible, with this dispenser, to pipette a volume of 1 µl to 10 mL. Finally, using ... read more
  • RNA Extraction Kit

    Tuesday, December 12, 2023
    I have used this RNA extraction kit many times on different cell types. This product is easy to use and extracts a correct amount of RNA. However, for every extraction, I could see during the nanodrop dosage that there was a lot of Guanidine contamination in my samples, which was coming from the kit... read more
  • Ham’s F-12K (Kaighn’s) Medium

    Tuesday, December 12, 2023
    I have been using this medium for the culture of my mesenchymal stem cells. The only negative point concerning this medium is the fact that it is necessary to add a lot of extra (SVF, Pen/Strep, Glutamax, Vitamine C, etc) in order for it to work fine. Other than that, this product is good and I have... read more
  • MiniAmp Thermal Cycler

    Tuesday, December 12, 2023
    I have been using MiniAmp Thermal Cycler during my dosages in my samples. This biosystem is small, compact and has a thermal range between 0 and 100 degrees. Moreover, this device has a touchscreen, which makes it easy to use and to program. I fully recommend it. read more
  • Polybrene Transfection Reagent

    Tuesday, December 12, 2023
    I have used the Polybrene Transfection Reagent to increase the adenovirus infection of osteoblasts. The Polybrene significantly increased the infection efficiency, even though the incubation time needs to be readjusted. read more
  • Cell Culture Medium

    Friday, December 08, 2023
    The StemPro Osteogenesis Differentiation Kit has proven very efficient in the differentiation stage of my mesenchymal stem cells into osteoblasts. Since my cells came from patients biopsies, they were more fragile once cultured. However, with the use of this specific medium, the cell viability was ... read more
  • iPS Culture

    Friday, December 08, 2023
    I used Geltrex for thawing and culturing induced pluripotent stem cells. This product has proven very efficient because the proliferation rate of the iPS was good and there was no uncontrolled differentiation. read more
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