Saurat Dione

Saurat Dione

Engineer

Articles by Saurat Dione

  • BSA Powder for Western Blot

    Monday, January 08, 2024
    I regularly use this BSA powder for my Western Blot experiment, and more specifically, during the blocking step, which allows the saturation of all non-specific antibody sites. It is very easy to prepare, by adding TBS and Tween. However, in my case, my antibody did not have very good specificity, ... read more
  • BMPR1B Antibody

    Monday, January 08, 2024
    I have been using this BMPR1B antibody recently for my western blot from protein extractions of osteoblasts. The dilution range goes from 1:500 to 1:2000 and in my case, I have been using it with a 1:1000 dilution. So far, this antibody has proven very efficient as it shows only my protein of ... read more
  • ATPlite Assay

    Monday, January 08, 2024
    I use this ATPlite Luminescence Assay system daily to dose the amount of ATP produced by my HEK cells. This kit is very easy to use and works perfectly. However, I would advise you to sample it with the right amount for each experiment because its efficiency decreases drastically if we thaw it too ... read more
  • Millex-GP 0,22 µm Filter

    Monday, January 08, 2024
    This Millex-GP 0,22 µm filter is very efficient in sterilizing all solutions we are using in cell culture. Indeed, this size is quite small, which prevents contamination of our medium. Moreover, this filter offers the possibility to screw it to different sizes of syringes, which allows you to adapt ... read more
  • DC Protein Assay Reagents Package

    Monday, January 08, 2024
    This DC Protein Assay is a colorimetric assay that I use often to determine protein concentrations. This kit provides the needed reagents, it is very easy to use, and does not require a lot of preparation. This colorimetric assay has proven very efficient in terms of determining protein ... read more
  • Power Pack for Gel Electrophoresis

    Monday, January 08, 2024
    I have been using this power pack to run my electrophoresis gel for a while. It is very easy to use and it works well, I have had no problems with the migration of my proteins on the gel. However, this device is very sensitive to the cabling and leaks within the gel support, which leads to an error ... read more
  • Mini-PROTEAN TGX Gels

    Monday, January 08, 2024
    Our lab has been using these precast gels quite often, which is time-saving because the gel preparation is a long process. Moreover, these gels go from 4% to 15%, which allows the separation of proteins between 5 and 200 kDa. It is possible to have a run time as short as 15 minutes with these gels ... read more
  • Cell Culture Media – MEM a

    Monday, January 08, 2024
    I have been using this MEMa media to differentiate monocytes into osteoclasts. In order to obtain a successful differentiation, I have to add antibiotics, Hyclone serum, B-Mercapto, RANKL, and MCSF to the media. So far, I have used this media every time and I can say that the cell viability was ... read more
  • Centrifugal Filters for Protein Purification

    Monday, January 08, 2024
    I have been using these centrifugal filters for a while now to purify my samples. These Amicon Ultra are very easy to use, can put up to 500 µl of samples in the filter and it can easily fit inside the Eppendorf Microcentrifuge. As far as I can tell, every test I have performed on these purified ... read more
  • Real-Time PCR System

    Monday, January 08, 2024
    I regularly use this Real-Time PCR System and I have to say that I really like it. I find it very easy to use, with an intuitive interface and it also offers an intuitive data analysis setting. So far, this system delivered accurate results, which are easily reproducible. However, I find it ... read more
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