Thippeswamy Sannasiddappa

Thippeswamy Sannasiddappa

Postdoctoral Research Fellow

Articles by Thippeswamy Sannasiddappa

  • Measurement of Bacterial Gene Expression by Fluorescence Method

    Wednesday, February 10, 2016
    I measured induction of the acid response gene called rcsB fused downstream of lux promoter which encodes for GFP (green fluorescent protein) in rcsB knock out strain background. I used Fluoroskan Ascent FL microplate Fluorometer. After growing E. coli cells to OD 600 of 0.3 in 200 µl volume in a 96... read more

  • Measurement of Quality and Quantity of DNA and RNA

    Monday, February 08, 2016
    Nanodrop ND-1000 spectrophotometer was used for determination of quality and quantity of nucleic acids particularly DNA and RNA from bacterial samples. After loading 1 µl of DNA or RNA sample on to Nanodrop, I got quality (260:280 ratio) of DNA at 1.82 to 1.85 with 40-50 ng/µl of DNA. For RNA ... read more

  • Quantification of Bacterial RNA Samples for RNA-Sequencing Experiments

    Monday, January 11, 2016
    I have extracted RNA from bacterial samples for next generation sequencing to study transcriptional response. Quantification of RNA samples using Nanodrop spectrometer is often regularly used. However Nanodrop spectrometer does not differentiate between DNA and RNA in the same sample leading over or... read more
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