Vincent Giacalone

Vincent Giacalone

Graduate Student

Articles by Vincent Giacalone

  • Easy to Use Instrument for Metabolic Assays, Some Weakness with Repeatability

    Tuesday, June 22, 2021
    The Seahorse XFp (and similar instruments for different plate sizes) can measure cellular metabolism with a variety of assay kits. In principle, cells are seeded in an 8-well plate and 4 test compounds can be sequentially injected into the well for calculating basal respiration, ATP production, ... read more

  • The Perfect Freezer Box For Any Freezer

    Thursday, June 03, 2021
    These Thin Film Freezer boxes are the best box I have used for freezer storage. They are 9x9 and all plastic. The thin wall of the box and lid made these boxes fit in a freezer rack that none of the other freezer boxes I had were compatible with. Unlike cardboard boxes they will not become damaged ... read more

  • Great Plate Reader for Almost Any Type of Measurement

    Wednesday, May 26, 2021
    the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme kinetics etc. It can perform endpoint measurements and time course readings and can inject precise volumes at ... read more

  • Electronic Pipette with Good Precision and Functions but One Flaw

    Friday, May 14, 2021
    the Xplorer plus electronic pipette for 15-300 uL is an all around useful tool. A variety of pipetting options can be selected such as dispensing fixed volumes, pipet + mix, manual, etc. It is comfortable to use for long periods of time and can fulfill many different roles. In that respect it does ... read more

  • Easy Way to Prepare Cells for Microscopy

    Monday, May 10, 2021
    The Cytospin is a great tool for preparing microscopy slides. It is a type of centrifuge that takes a cell suspension loaded in a funnel and deposits the cells into a precise circular area for easy viewing. Because the cells are concentrated into a small area, few cells are needed. I routinely ... read more

  • Freezing Chamber for Cryopreservation of Primary Cells and Cell Lines

    Tuesday, May 04, 2021
    This freezing chamber is a great product for reliable cryopreservation of all cell types. After resuspending cells in desired freezing media, the cryovials are placed in the chamber and any empty spots should be filled in with tubes containing an equal volume of water. The Cool Cell is then placed ... read more

  • Excellent Cytokine Multiplex Detection Platform

    Tuesday, April 27, 2021
    The MesoScale cytokine multiplex assay is a great platform for quantifying many types of protein targets in biological fluids. The Quickplex SQ120 reader is required for reading these electrochemiluminescent plates. These assays offer approximately 5 logs of linear range of measurement. The SQ120 ... read more

  • Powerful Imaging Microscope, but Not Easy to Learn

    Thursday, April 22, 2021
    We acquired this microscope to fulfill several purposes, including cell counting and climate controlled expriments using both blood leukocytes (neutrophils, monocytes) and epithelial cells. Specific objectives included assessing caspase activity over time following viral infection, quantifying cell ... read more
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