An HPLC detector is used to analyze the components of the eluted mixture that is collected after being run through an HPLC column. Differences in detector type are based on what is being detected. Detection can be done by measuring absorbance, changes in reflex indices, molecular weight, and fluorescence among others. When selecting a detector, it is important to consider the sample type being analyzed. Some sample types will not work or will have lower sensitivity on particular detectors. Also of consideration is the type of information that is being sought by the analysis and what method will be used when running the column, for example, some detectors will work with the gradient method while others will not.
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| Company | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology |
| Item | Optilab™ Refractive Index Detector for HPLC/GPC | Charged Aerosol Detector (CAD) | omniDAWN™ Multi-angle Light Scattering (MALS) Instrument |
| Citations | | | |
| Catalog Number | Optilab | CAD | omniDAWN |
| Price | | | |
| Type | Refractive Index Detector | Inquire | Multi-Angle Light Scattering (MALS) photometer |
| Detector(s) | Differential Refractive Index (dRI) | Inquire | Inquire |
| Applications | GPC/SEC analysis of macromolecules by SEC-MALS | Inquire | With standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio, and particle concentration. With the Calypso composition-gradient system: label-free characterization of biomolecular interactions, including self- and hetero-association as well as non-specific interactions. |
| Description | The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same wavelength as the MALS detector, for maximum accuracy. With an operating window from 4°C - 65°C it is appropriate for both protein and polymer samples. Key benefits include its extraordinary range: -0.0047 to +0.0047 RIU (comparable to 25 mg/mL protein in PBS); the high-concentration version extends the range by 7x. Its sensitivity is ± 7.5 x 10-10 RIU across the entire measurement range. It also includes a high-resolution, touch-screen front panel that displays data graphs and diagnostics.... Read More | The Waters Charged Aerosol Detector (CAD) delivers reliable, near-universal detection that helps biopharmaceutical scientists quantify a wide variety of components involved in modern drug development. Its ability to provide a consistent response for molecules that lack strong UV absorbance makes it The Waters Charged Aerosol Detector (CAD) delivers reliable, near-universal detection that helps biopharmaceutical scientists quantify a wide variety of components involved in modern drug development. Its ability to provide a consistent response for molecules that lack strong UV absorbance makes it especially valuable when working with complex formulations, excipient mixtures, and process-related impurities. With robust performance and straightforward operation, the CAD supports confident decision-making from early analytical exploration through late-stage process control.
Key Features and Benefits:- Universal detection performance for compounds that are traditionally difficult to monitor with UV alone, including lipids, sugars, and surfactants.
- High sensitivity and low detection limits to strengthen impurity tracking and enhance characterization of challenging components.
- Broad dynamic range enabling simultaneous measurement of major constituents and trace-level species.
- Complementary to UV and mass detection, adding depth and flexibility to method development strategies.
- Fully compatible with Waters UPLC, UHPLC, and HPLC systems, with Empower™ Software that supports efficient, compliant data analysis across biopharma workflows.
... Read More | Delivering up to 4× faster runs with half the sample, the omniDAWN™ multi-angle static light scattering (MALS) photometer is purpose-built for UPLC™ and UHPLC systems for non-destructive absolute molar mass and size characterization of macromolecules and nanoparticles in solution. It provides Delivering up to 4× faster runs with half the sample, the omniDAWN™ multi-angle static light scattering (MALS) photometer is purpose-built for UPLC™ and UHPLC systems for non-destructive absolute molar mass and size characterization of macromolecules and nanoparticles in solution. It provides exceptional sensitivity across a broad range of molecular weights, sizes, and concentrations, supported by advanced analysis modules, practical implementation guides, and intelligent system health monitoring to ensure reliable performance and streamlined workflows.... Read More |
| Wavelength Range | Inquire | Inquire | Inquire |
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