When delivering smaller particles through the liquid mobile phase of a HPLC unit, the liquid must be forced through the column under pressure. The purpose of an HPLC pump, also called a solvent delivery system, is to force the liquid through the system while maintaining a specific flow rate. HPLC pumps can be broadly categorized into three types: syringe pumps that provide pulseless delivery, reciprocating or piston pumps that push the mobile phase through with a series of pistons, and pneumatic pumps that use gas to pressurize the mobile phase. Reciprocating pumps are most common due to their good pressure generation, constant flow rate, and small footprint, but each type of pump has its pros and cons that should be weighed when purchasing. Other things to look out for when selecting a pump include the instrument’s compatibility with different solvents, low dead volume to minimize issues with solvent changeover, delivery method (either isocratic or gradient), as well as things like price, ease of use, and lifetime.
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| Company | Shimadzu | Shimadzu | Shimadzu | Shimadzu | Shimadzu |
| Item | LC-20AP Large-scale Solvent Delivery Unit | i-Series LC-2080 | LC-40B X4 UHPLC Pump | LC-40D HPLC/UHPLC Pump | LC-40D XR HPLC/UHPLC Pump |
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| Catalog Number | LC-20AP | 228-65883-58 | | LC-40D | |
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| Description | The next generation LC-20AP Preparative LC Pump can be used for analytical, semi-prep and prep LC applications without changing pump heads.
The wide flow rate range (0.01 - 150mL/min) with high pressure tolerance (6,000 psi up to 100mL/min and 4,350 psi up to 150mL/min) makes it possible to use The next generation LC-20AP Preparative LC Pump can be used for analytical, semi-prep and prep LC applications without changing pump heads.
The wide flow rate range (0.01 - 150mL/min) with high pressure tolerance (6,000 psi up to 100mL/min and 4,350 psi up to 150mL/min) makes it possible to use 50mmID x 250mmL 5um-particle size columns to achieve high-resolution and high-efficiency purification, and improve productivity by five to ten times over the previous models. The flow rate accuracy and precision were drastically improved by minimizing dead volume in the pump heads and using higher resolution micro-stepping motor control so that the pump can be used at an analytical flow rate range, such as 1mL/min, with high reliability.
Overall productivity and quality of the purification has also been greatly improved. If configured as an analytical/prep system, a user can develop a method at analytical scale then immediately convert the method for prep scale. Shimadzu provides a tool to easily convert methods between analytical and prep for support customers. In addition, the LC-20AP pump can be used with Open Solution automated prep LC/MS software, which allows researchers to purify their compounds by simplifying the workflow in a walk-up environment.
In addition, the LC-20AP consumes 40% less energy in comparison with the previous model thanks to a newly designed high-efficiency power supply and motor control electronics. This makes the product more ecological.... Read More | Inquire | Inquire | The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. The dual piston design has minimal pulsation for stable baselines.
Features: - Operable in constant flow or constant pressure mode, as well as isocratic, low pressure
The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. The dual piston design has minimal pulsation for stable baselines.
Features: - Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
- Operable in constant flow or constant pressure mode, as well as isocratic, binary high-pressure gradient, ternary high-pressure gradient or quaternary low-pressure gradient mode.
- Internal solvent selection valve (2 or 4-channel) or a low-pressure gradient formation unit (LPGE) can be added as options.
- A rinse kit can be added to wash behind the seals to minimize salt precipitation if buffered mobile phases are used.
- Users can set compressibility to further reduce pulsation during operation.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
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| Type | Series-Type Double Plunger | UHPLC System (integrated, compact) | UHPLC Pump | Parallel-type double plunger | HPLC/UHPLC Pump |
| Plunger Capacity | 250 ul | Inquire | Inquire | Inquire | Inquire |
| Flow Rate | 0.01 to 150.0 mL/min | 0.0001 to 10 mL/min | 0.0001 to 2.0000 mL/min | 0.1 uL/min to 10 mL/min | 0.1 µL/min to 10 mL/min |
| Gradient Type | Constant-Pressure Solvent Delivery | Quaternary low-pressure gradient (LPG) | Binary high-pressure gradient | High-pressure or quaternary low-pressure | Isocratic, binary HPG, ternary HPG, or quaternary LPG |
| Number of Mixed Solvents | Inquire | 4 | 2 | Inquire | 4 |
| Max. Pressure | 42.0 Mpa | Inquire | Inquire | 44 Mpa | Inquire |
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