| Description | Neural stem cells (NSC) are neural progenitor cells that can differentiate into different types of cells in the nervous system under certain conditions. Taking advantage of their continued reproduction, NSC has great potential for research in neurogenesis, neurodegenerative diseases, and clinical Neural stem cells (NSC) are neural progenitor cells that can differentiate into different types of cells in the nervous system under certain conditions. Taking advantage of their continued reproduction, NSC has great potential for research in neurogenesis, neurodegenerative diseases, and clinical transplantation applications. NeuroShop's human neural stem cell line is derived from human-induced pluripotent stem cells, which are produced from adult skin fibroblasts using a unique, non-footprint free iPSC reprogramming method. When cultured in a medium containing bFGF and EGF, these cells can normally proliferate without differentiation into neuronal cells.
Main features
Derived from human iPS cells without footprints
Homogeneous population
Under certain conditions, it can differentiate into more than 90% of neurons... Read More | Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the P301L mutation (CCG>CTG) into the MAPT gene. This line is homozygous for the P301L mutation so both alleles contain the mutation. Click on the product images to see the data and Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the P301L mutation (CCG>CTG) into the MAPT gene. This line is homozygous for the P301L mutation so both alleles contain the mutation. Click on the product images to see the data and further details.
The P301L mutation MAPT has been implicated in frontotemporal dementia and parkinsonism (Dumanchin et al., 1998). The mutation affects only the 4R isoforms of MAPT since the exon containing the mutation is spliced out of 3R isoforms (Hutton et al., 1998). Aggregated MAPT/tau protein in affected patients consisted mainly of the 4R isoform (Hutton et al., 1998). The P301L mutation promotes aggregation of MAPT/tau protein into ordered paired helical filaments and beta sheet formation in vitro (von Bergen et al., 2001).... Read More | iNeuMito™ biosensor is a mitochondrial calcium flux sensor. These cells are engineered to express iNeuMito™ biosensor, which has been developed to use a highly sensitive fluorescence-based biosensor as a reporter gene to monitor mitochondrial calcium flux and cytotoxicity. Excitation = iNeuMito™ biosensor is a mitochondrial calcium flux sensor. These cells are engineered to express iNeuMito™ biosensor, which has been developed to use a highly sensitive fluorescence-based biosensor as a reporter gene to monitor mitochondrial calcium flux and cytotoxicity. Excitation = 610nm; emission = 625nm. The iNeuMito™ biosensor is introduced into the cell using a proprietary magnetic liposome-mediated method and expressed in the mitochondria (using a marker protein such as COX IV).
iNeuATP™, a fluorescent biosensor, has also been custom incorporated into the human iNeuRapid™ cell line. With iNeuATP™, biosensor assays provide fast kinetics (resolution in seconds) when measuring real-time cellular ATP metabolism and cytotoxicity. The analysis of cellular oxidative phosphorylation directly reflects the health and function of mitochondria. Excitation = 535nm; emission = 550nm.
Starting Materials: immortalized human cell line.... Read More | This product GTTS-WQ7127MR is a type of mRNA having 120 nt poly(A) tail and modified with Cap 0 & N1-Methylpseudo-UTP. It ecodes the monoclonal antibody that targets LHCGR gene. The antibody can be applied in Controlled Ovarian Stimulation (COS) research. | Creative Biolabs' Neuropathy Frozen Tissue Panels are designed for the study of Alzheimer's disease, Parkinson's disease, dementia, multiple sclerosis, progressive supranuclear palsy (PSP) and depression. |