Cell Sorter

Cell Sorter Cell sorters are specialized laboratory instruments used to identify and separate specific species or populations of cells within a sample. Automated cell sorting provides high throughput sample preparation for downstream analysis within the life science and pharmaceutical industries. Many cell-sorting instruments also reduce the risk of cross-contamination and operator exposure by containing the samples within a cartridge or chip. Cell sorters employ several methods to achieve accurate and gentle sorting, including fluorescence activated cell sorting (FACS), Magnetic Activated Cell Sorting (MACS), and various microfluidic or on-chip sorting methodologies. FACS cell sorting relies on the addition of fluorescent targets to a cell suspension, while MACS utilizes magnetic beads to bind to target cells. Microfluidic cell sorters can be especially useful for the enrichment of target cell types, however, these typically require a larger cell concentration and volume. When comparing cell sorters, consider which methodology would work best for your assay or sample type. Additionally, flow rates and throughput can vary from one instrument to another.

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CytoFLEX SRT Benchtop Cell Sorters
Beckman Coulter Life Sciences
  • Sample Volume:
    Inquire
  • Flow Rate:
    10 to 100 µL/min
  • Throughput:
    40,000 particles/sec; 30,000 particles/sec (sorting)
MoFlo Astrios Cell Sorters
Beckman Coulter Life Sciences
  • Sample Volume:
    2-way sorting: 1.5, 5, 15 and 50 mL tube sizes (optional) 4-way sorting: 3x5 mL plus 1x50 mL (optional) 6-way sorting: 1.5 (optional) and 5 mL tube sizes 6, 24, 96, 384 and 1,536-well plates, custom plates
  • Flow Rate:
    Inquire
  • Throughput:
    100,000 events/second; 70,000 sortings/second; 2-way sorting, 4-way sorting, 6-way sorting
MACSQuant&reg; Tyto&reg; Cell Sorter
Miltenyi Biotec
  • Sample Volume:
    100 µL to 10 mL
  • Flow Rate:
    up to 8mL/h
  • Throughput:
    up to 110,000 cells/s
Citations:
(2)
Bigfoot™ Cell Sorter
Thermo Fisher Scientific
  • Sample Volume:
    User-configurable sort output holders for 1.5, 5, 15, and 50 mL tubes; microwell plates of up to 1,536 wells; microscope slides; and even 10x™ chips with integrated temperature control (4–37°C)
  • Flow Rate:
    Inquire
  • Throughput:
    >70,000 EPS
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  • Miltenyi Biotec
  • Thermo Fisher Scientific

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