Time-of-flight mass spectrometers (TOF MS) do exactly what their name suggests: determine an ion’s mass-to-charge ratio (m/z) based on how long it takes for that ion to travel down the instrument’s “flight tube” and reach the detector. Frequently, though not exclusively coupled to matrix-assisted laser desorption ionization (MALDI) sources, TOF MS come in a variety of configurations, including a single-stage time-of-flight mass spectrometer, a multi-stage TOF/TOF, and even a hybrid quadrupole-TOF (Q-TOF) instrument; the latter two enable MS/MS applications. Often, time-of-flight mass spectrometers contain two detectors: a linear detector at the end of the flight tube, and a “reflector” detector, which captures ions that were redirected back towards the source by a “reflectron.” The reflectron serves two purposes: correcting for discrepancies in initial kinetic energy of identically sized molecules (which would cause them to reach the detector at different times and thus, have different apparent m/z values) and lengthening the flight path, improving resolution.
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LECO
- Electron Ionization
- Time of Flight
- Up to 50,000 FWHM at 200 spectra/second
- Inquire
LECO
- Electron Ionization
- Time of Flight
- Up to 50,000 FWHM
- Inquire
Other Time of Flight Mass Spectrometer (TOF MS) Products
LECO
- Electron Ionization (EI)
- Time of Flight
- Inquire
- Inquire
JEOL USA, Inc.
- FI, FD
EI, PI, CI optional - TOF
- > 30,000 FWHM
- Up to m/z 6,000
SCIEX
- Turbo V ion source with twin sprayer ESI probe
and twin sprayer APCI probe - QTOF
- Equal to or greater than 42,000 (FWHM) measured on the (M+6H)6+ charge isotope cluster for bovine insulin at m/z 956
- TOF: 40 kDa
Precursor Ion Selection: 5–2250 m/z
SCIEX
- APCI and ESI modes
- TOF
- Equal to or greater than 42,000 (FWHM) measured on the [M+6H]6+ charge isotope cluster for bovine insulin at m/z 956
- TOF: 40 kDa
Precursor Ion Selection: 5–2250 m/z
Shimadzu
- MALDI
- TOF
- >5000 FWHM
- 1 kDa to 500 kDa
Shimadzu
- MALDI
- TOF
- 5000 FWHM in linear mode; 15000 FWHM in reflectron mode
- 1 kDa to 500 kDa
Shimadzu
- MALDI
- TOF/TOF
- >20,000 FWHM (Reflectron mode)
- 1 kDa to 500 kDa
Shimadzu
- ESI, APCI, DUIS, Micro
- Quadrupole Time-of-Flight (Q-TOF)
- 30,000 FWHM
- m/z 10 to 40,000
Shimadzu
- ESI, APCI, DUIS, Micro
- Quadrupole Time-of-Flight (Q-TOF)
- Inquire
- Inquire
Shimadzu
- MALDI
- TOF
- 180 to 200 kDa
- 0.01 to 1.5 Mda
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