MALDI TOF mass spectrometers (matrix-assisted laser desorption/ionization/time-of-flight) are amongst the most popular mass spec instruments in biology. They combine two technologies, the “soft” MALDI ionization source and the TOF mass analyzer. The latter measures an ion’s mass-to-charge ratio (m/z) by measuring the time it takes to reach a detector. The former volatilizes and ionizes those molecules using a laser, a target, and an organic compound called a matrix. In MALDI, a sample (for instance, a collection of tryptic peptides) is mixed with matrix, spotted and dried (crystallized) on the surface of a metal target plate. Upon irradiation with laser light, the matrix absorbs the energy and converts it to heat, which then causes the sample to ionize and explode off the plate into the mass analyzer. MALDI TOF mass spectrometers come in two basic flavors, MALDI TOF MS and MALDI TOF/TOF MS; the latter enables tandem MS (MS/MS) studies.
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Shimadzu
- MALDI
- TOF/TOF
- >20,000 FWHM (Reflectron mode)
- 1 kDa to 500 kDa
Shimadzu
- MALDI
- TOF
- 5000 FWHM in linear mode; 15000 FWHM in reflectron mode
- 1 kDa to 500 kDa
Shimadzu
- MALDI
- TOF
- 180 to 200 kDa
- 0.01 to 1.5 Mda
Shimadzu
- MALDI
- MALDI-TOF MS
- Inquire
- Inquire
Shimadzu
- MALDI
- TOF
- >5000 FWHM
- 1 kDa to 500 kDa
Shimadzu
- MALDI
- TOF
- >5000 FWHM – ACTH 18-39 (m/z 2465)
- m/z 1 - 500,000
Shimadzu
- MALDI
- Time-of-Flight
- Inquire
- m/z 1,000–1,500,000Da
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