Comprising two components, a liquid-phase separation system and a mass spectrometer, liquid chromatograph mass spectrometers (LC MS instruments) couple sample fractionation and analysis. The separation can be accomplished via standard liquid chromatography, HPLC, UPLC, or even nano-LC. Any liquid-phase separation will do, so long as its output can be directed into the mass analyzer (for instance, via an electrospray ionization source). The mass spectrometer is similarly flexible, ranging from the simplest single quadrupole to a Fourier transform ion cyclotron resonance instrument. Just remember, in a liquid chromatograph mass spectrometer the column flow is continuous, meaning the mass analyzer must be fast enough to thoroughly analyze one peak before the next one comes off the column. And, unlike with MALDI MS, once a peak is analyzed, it’s gone.
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Shimadzu
- ESI, APCI, Dual Ionization, Micro-ESI
- Q1 & Q3 are molybdenum hyperbolic mass filters with pre-rods; Q1 includes post-rods
- Max. 30,000 u/sec (in all scan modes)
(0.1 u step: 300,000 data points/sec)
- R < 0.7 u FWHM and adjustable to 0.5 u
SCIEX
- OptiFlow® Pro Ion Source
- Triple quadrupole
- 20,000 Da/s
- Up to 3100 for mass 922 in LIT mode 1000 Da/s (0.3 Da)
Tune to a peak width of 0.5 Da for TQ mode
Thermo Fisher Scientific
- ESI and HRAM
- Orbitrap
- Up to 22 Hz at resolution setting of 15,000 at m/z 200
- Up to 240,000 at m/z 200
Thermo Fisher Scientific
- ESI and HRAM
- Orbitrap
- Up to 40 Hz at resolution setting of 7500 at m/z 200
- 480,000 at m/z 195
Thermo Fisher Scientific
- Inquire
- UHMR Hybrid Quadrupole-Orbitrap™
- up to 12 Hz at resolution setting of 12,500 at m/z 400
- 200,000 at m/z 400
Shimadzu
- ESI, APCI, Dual Ionization
- Q1 & Q3 are molybdenum hyperbolic mass filters with pre-rods; Q1 includes post-rods
- Inquire
- Inquire
SCIEX
- Electrospray Ionization
- Triple Quad or TOF
- Up to 3 samples per second
- Inquire
Shimadzu
- ESI, APCI, Dual Ionization, Micro-ESI
- Q1 & Q3 are molybdenum hyperbolic mass filters with pre-rods; Q1 includes post-rods
- 30,000 u/sec
- R < 0.7 u FWHM
SCIEX
- APCI and ESI modes
- TOF
- up to 25 Hz (25 spectra per second)
- Equal to or greater than 42,000 (FWHM) measured on the [M+6H]6+ charge isotope cluster for bovine insulin at m/z 956
Thermo Fisher Scientific
- Inquire
- Orbitrap
- OTMSn up to 40 Hz ITMSn up to 45 Hz
- 7500 to 500,000 (FWHM) at m/z 200
Thermo Fisher Scientific
- HESI
- Orbitrap
- Inquire
- 7500-500,000 (FWHM) at m/z 200
SCIEX
- Turbo V ion source with twin sprayer ESI probe
and twin sprayer APCI probe - QTOF
- Up to 25 Hz (25 spectra per second)
- Equal to or greater than 42,000 (FWHM) measured on the (M+6H)6+ charge isotope cluster for bovine insulin at m/z 956
Thermo Fisher Scientific
- ESI
- Orbitrap
- Inquire
- 7500 to 500,000 (FWHM) at m/z 200
Waters | Wyatt Technology
- Inquire
- Inquire
- Inquire
- Inquire
Shimadzu
- ESI, APCI, Dual Ionization
- Single Quadrupole
- 15,000 u/sec
- R = 2 M
Shimadzu
- ESI, APCI, Dual Ionization, Micro-ESI
- Q1 & Q3 are molybdenum hyperbolic mass filters with pre-rods; Q1 includes post-rods
- Max. 30,000 u/sec (in all scan modes) (0.1 u step: 300,000 data points/sec)
- R < 0.7 u FWHM and adjustable to 0.5 u
Shimadzu
- Micro-ESI
- Q1 & Q3 are molybdenum hyperbolic mass filters with pre-rods; Q1 includes post-rods
- Inquire
- Inquire
Shimadzu
- ESI, APCI (optional)
- Q1 & Q3 are molybdenum hyperbolic mass
filters with pre-rods; Q1 includes post-rods
- Max. 30,000 u/sec (in all scan modes)
- R < 0.7 u FWHM and adjustable to 0.5 u
Thermo Fisher Scientific
- Inquire
- Inquire
- Inquire
- Inquire
Gilson Inc
- Inquire
- Inquire
- Inquire
- Inquire
Gilson Inc
- Inquire
- Inquire
- Inquire
- Inquire
JEOL USA, Inc.
- ESI, DART, and CSI (ColdSpray) are supported
- Time-of-Flight
- Up to 16,000 spectra/second
- Greater than 6,000 FWHM (Full Width at Half Maximum)
Thermo Fisher Scientific
- Inquire
- Inquire
- Up to 22 Hz
- Up to 120,000 (FWHM) at m/z 200, demonstrates outstanding resolving power, according to a product page
SCIEX
- icIEF
- Time of Flight
- Inquire
- Inquire
Shimadzu
- ESI, APCI, Dual Ionization
- Single Quadrupole
- 15,000 u/sec
- Inquire
Thermo Fisher Scientific
- ESI and HRAM
- Orbitrap
- Up to 22 Hz at resolution setting of 15,000 at m/z 200
- Up to 120,000 at m/z 200
PerkinElmer
- ESI, APCI
- Triple Quadrupole
- Inquire
- Inquire
Inquire
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