Next-generation sequencing, also known as next-gen sequencing, NGS, or High-throughput sequencing, is a revolutionary tool in genomic and life science research. For NGS library construction, preparation of the target nucleic acid, DNA or RNA, into a compatible form for the sequencing system is fundamental. Usually, the main process in preparing RNA or DNA for next-gen sequencing analysis involves fragmenting and/or sizing the target sequences to their desired length and then converting the target to double-stranded DNA. The final step involves attaching oligonucleotide adapters to the ends of the fragments, and quantifying the final product for sequencing.
There are several things to consider when preparing libraries from samples including sample applications, equipment options (low or high-throughput), budget, and workflow.
Companies that sell Next-Gen Sequencing Library Construction / Fragment Library System
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