A UV-Vis spectrophotometer is used to determine the absorption of light from a sample and can be used as a detector for HPLC. A sample is placed in the UV/VIS beam and absorbance versus wavelength is measured. A double beam spectrophotometer utilizes two beams of light: a reference beam and a sampling beam that passes through the sample. Some double beam spectrophotometers have two detectors that allow the two beams to be measured at one time.
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Thermo Fisher Scientific
- UV-Visible
- 190 nm to 1100 nm
- ± 0.5 nm
- Xenon Flash Lamp (>5 years typical, 3 years guaranteed)
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