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Ready to Use Kanamycine

Filippo Massa
We perform a lot of cloning, so we need a lot of Kanamycine for the bacteria selection and growth. This formulation is already at classical concentration to be readily diluted... read more
Magnets for Eppendorf Tubes

Filippo Massa
Magnetic beads based protein purification can be very tricky with small volumes. This magnet can arrange up to 6 Eppendorf tubes (1,5 and 2 ml), allowing the process of ... read more
Excellent Antibiotic for Bacteria Selection

Filippo Massa
Performing a lot of cloning, we tested a lot of antibiotics. This reference of Ampicillin works very well and it is often in offer, with nice reductions. Budget restrictions ... read more
Good Quality PCR Plates

Neha Mittal
The skirted PCR plates are good to run samples at even high temperatures. It didn't spill the samples out or mix into other wells while handling the plates due to its firmness... read more
Cell Line Freezing Container

Ganesh Pusapati
This is an 18-slot cryovial freezing container. I typically use 2-ml cryovials for freezing my cell lines. I place cryovials in this container (filled with fresh isopropanol) ... read more
X-tremeGENE 9 for Mammalian Cell Transfection

Ganesh Pusapati
X-tremeGENE 9 is a well established transfection reagent for mammalian cell lines. I use this reagent at 1:3 (DNA:transfection reagent) for most of my experiments. The ... read more
X-tremeGENE HP Transfection Reagent

Ganesh Pusapati
I have been using this product for over a decade for hard-to-transfect cell lines. I typically use this transfection reagent at 1:3 (DNA:reagent). I have noticed that this ... read more
siRNA Transfection Reagent

Ganesh Pusapati
I use this product for transfecting siRNA in mammalian cell lines. I typically use it at 1:3 (siRNA:transfection reagent). I have tested it in HeLa and 3T3 cell lines and did ... read more
Genomic DNA Extraction Reagent

Ganesh Pusapati
I use this reagent for extracting genomic DNA from cell lines. I typically use this reagent at 3-5x volume of cell pellet size. I strictly follow the manufacturer's ... read more
Transfection Reagent for 293-F Cell Line

Ganesh Pusapati
I use this reagent for transfecting 293-F (suspension format) cell lines. I use the reagent at 3-4x of DNA amount (µL reagent: µg DNA). This is a great reagent for protein ... read more
Transfection Reagent for Insect Cell Expression

Ganesh Pusapati
I use this reagent to transfect recombinant bacmid into Sf9 (insect) cells to generate P0 baculovirus. I typically seed 1 million cells per well in a 6-well plate and ... read more
Good Method to Study Mitochondrial Respiration

Lisha Joshi
The energy metabolism of microglia activated with lipid biomolecule was measured using Agilent Seahorse XF analyzer. Serial addition of inhibitors of mitochondrial electron ... read more
Easy Glycolysis Assay Kit

Lisha Joshi
Energy metabolism of microglia were studied with glycolysis assay kit. This kit measures the lactate secreted in the media by the cells treated with various substances. The ... read more
Assessing sGAG Using the Blyscan™ - sulfated Glycosaminoglycan (sGAG) Assay Kit

Rachel Crossland
I have used the Blyscan™ - sulfated Glycosaminoglycan (sGAG) assay kit to assess GAG in chondrocytes isolated from osteoarthritis patients. The sGAG levels in the samples were... read more
Visualization of Nuclei Using DAPI

Rachel Crossland
I have used the Fluoromount-G™ Mounting Medium, with DAPI to visualize nuclei. This was for human chondrocytes. I followed the manufacturer's instructions and visualized the ... read more