May 29, 2012 - The New AgilePulse® In Vivo Electroporation System possesses unique, variable pulse amplitude technology for vaccine delivery or gene therapy applications.
Patented Pulse Agile™waveforms consists of multiple, low to high voltage pulses with variable pulse lengths, which promotes greater cell membrane poration; resulting in maximum transfection efficiency with minimal damage to the cells or tissues. Gene expression in skin is 100-fold higher1 when delivery is enhanced by electroporation compared to injection alone.
Features:
- Patented Pulse Agile® technology
- Resistance measurement for proper needle placement and reproducibility
- Specially designed needle arrays for intra-muscular or intra-dermal applications
Benefits:
- Enhanced immune response1
- Increased Ag-specific T-cell response1
- Gene expression is 100-fold higher1
Figure 1. Time kinetics of transgene expression in skin after DNA electrovaccination. (a) Time course of in vivo luciferase expression after intradermal (i.d.) DNA delivery alone (dotted line) and after i.d. DNA delivery followed by electroporation (filled line). One representative experiment of two is shown (n = 8). (b–d) Immediate monitoring of gene expression after DNA electrovaccination. Representative bioluminescent images showing luciferase expression in skin at different time points after DNA electrovaccination. N denotes the negative control (non-injected). The scale shows intensity of luminescence (photons/sec/cm2). The experiment was repeated three times.
About BTX
BTX is a global provider of novel electroporation and electrofusion tools for the advancement of cell transfection and cell fusion. BTX products continue to sustain scientific research in academia, biotech, agriculture and pharmaceutical laboratories. From the introduction of our line of specialty electrodes for in vivo electroporation, to our ground breaking contribution to the field of high throughput and large volume transfection, to our involvement in the application of electric fields for hybridoma production and nuclear transfer cloning, BTX remains at the forefront of this cutting edge technology for which new applications are continually evolving.
Visit the BTX website at www.btxonline.com for more information on products, protocols or to view video tutorials for a variety of applications.
To learn more about this exciting new technology or other BTX electroporation or electrofusion products, contact:
BTX
84 October Hill Road
Holliston, Massachusetts 01746 USA
Toll Free: 800-272-2775
Phone: 508-893-8999 ext 167
Fax: 508-429-5732
E-mail: [email protected]
Web: www.btxonline.com