18th JULY 2013: MagSi-DNA cleanFIX from AMSBIO offers a cost effective solution for purifying DNA fragments, removing all unwanted side products and reagents, such as single nucleotides, terminator dyes, primers and primer dimers, enzymes, buffers and salts.
In many genomics applications, purification of DNA fragments is required to prevent downstream interference with analysis methods. Purification of PCR reactions often requires removal of dNTPs, primers, enzymes, salt by-products and various additives. Historically purification protocols have involved different sample types, different volumes and often multiple washing and centrifugation steps. Using such methods the errors incurred during sample preparation have resulted in lost time and significant costs due to re-work and lost reagents.
MagSi-DNA cleanFIX enables a combination of PCR clean-up and dye terminator removal from sequence reaction mixes with a single product. The kit uses magnetic silica bead technology, and is easily automated because no columns or centrifugation steps are involved. The kit uses a simple "Bind, Wash & Elute" procedure common to magnetic particle purification protocols.
Independent test results demonstrate how MagSi-DNA cleanFIX purification methods developed for a commercial liquid handling workstation provide an automated walk-away solution for PCR clean-up and dye terminator removal from sequence reactions. The precision and reproducibility of the workstation in combination with the customized magnets for MagSi-DNA cleanFIX are shown to allow fast magnetic separation and homogenization of the samples. Overall the PCR clean-up protocol using MagSi-DNA cleanFIX is shown to enable fast and efficient recovery of DNA fragments larger than 80 bp with >99% removal of primers and primer dimers. In addition the dye terminator removal method consistently delivered high quality sequence data with Phred >20 scores above 700.
For further information on MagSi-DNA cleanFIX and the fully range of magnetic beads available from AMSBIO please visit www.amsbio.com/DNA-sequencing-magnetic-purification.aspx or contact AMSBIO now on +44-1235-828200 / +1-949-768-8365 or email [email protected].
Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading company contributing to the acceleration of discovery through the provision of cutting-edge life science technology products and services for research and development in the medical, nutrition, cosmetics and energy industries. The AMSBIO range includes specialist antibodies, peptides and recombinant proteins. In addition the company is able to draw upon in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. Widely acknowledged as experts in cell culture, AMSBIO partners with clients in tailoring cell systems to enhance screening outcomes and eventual prognosis. With a range of molecular detection reagents, and a significant Biorepository the company can also provide tissue DNA, RNA, protein and microarray products. Key research areas for these products include: Oncology, Regenerative Medicine, Environmental Analysis, Cytotoxicity Screening, Glycomics and Stem Cell Biology.
AMS Biotechnology (AMSBIO)
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Oxon OX14 4SE
Email: [email protected]
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Lake Forest, CA 92630
Email: [email protected]