The Ab was used to detect changes in LPAR6 expression in untreated or LPS-treated (20 ng/mL for 24 h) BV-2 cells. It did work as expected, observing a band at the correct MW. However, in our case we also had some unspecific bindings at higher molecular weights. Apart from that, the antibody is quite... read more

As part of our study, we sought to investigate changes in LPAR2 expression after treatment with LPS. Even though the Ab reactivity is against human, we successfully used it to detect LPAR2 in a murine microglial cell line. Even though it has to be used at a 1:250 dilution, the Ab gives only one ... read more

The antibody was used to detect changes in the expression of synaptophysin in a mouse model of neuroinflammation using immunoblotting. The antibody was used previously for IHC experiments, providing really clear staining with minimal background. For the western blot experiments, we observed only one... read more

The specific antibody is very popular, being extensively used in immunofluorescence experiments as a marker of microglia activation. For the current experiments, we wanted to assess changes in Iba1 expression in mouse brain tissue using immunoblotting. Having worked flawlessly in IF experiments, the... read more

The antibody was used to detect changes in COX2 expression in mouse brain tissue using immunoblotting. Our aim was to study the effect of ATX inhibition (PF-8380) in different inflammatory markers. The antibody has been used numerous times in our lab, for assessing the expression of COX2 in samples ... read more