I use Sony Sorter for bulk and single cell sorting of mammalian cells. I typically use this 100 micron chip for most of my cell line sorting purposes. The chip is good for 24 hours and the installation process is easy. read more

I have been using this product for over a decade for hard-to-transfect cell lines. I typically use this transfection reagent at 1:3 (DNA:reagent). I have noticed that this reagent yields higher transfection efficiency compared to X-tremeGENE 9. I prefer to use this reagent for large size plasmid ... read more

I use this reagent for extracting genomic DNA from cell lines. I typically use this reagent at 3-5x volume of cell pellet size. I strictly follow the manufacturer's instructions and it works well. If undiluted genomic DNA needs to be used for PCRs, I recommend the genomic DNA not exceed 1/5th volume... read more

I use this reagent to transfect recombinant bacmid into Sf9 (insect) cells to generate P0 baculovirus. I typically seed 1 million cells per well in a 6-well plate and transfect after 1 h. I use 2 µg bacmid and 6 µl of this transfection reagent for this format and it works well. There is no toxicity ... read more