I have been using this centrifugal filter unit for more than 15 years. I typically use this unit for my recombinant protein purification process during the sample concentration step. Typical volumes that I work on are between 10-30 mL and my protein sizes >100 kDa. Easy to use and the best product ... read more

I typically use this resin when my recombinant His-tagged protein purification process requires DTT and EDTA in my binding buffer. This resin is different from the traditional Ni-NTA Agarose resins in that imidazole concentration for the wash and elution steps is low (1-5 mM for the wash step and 25... read more

I use these Tips for all my plasmid midi prep purifications. Culture volumes are 100 mL for low-copy number plasmids and 50 mL for high-copy number plasmids. Typical yields are 100-500 micrograms based on the plasmid copy number. I strictly follow manufacturer's instructions and never had any ... read more

I routinely resolve PCR products on agarose gels followed by gel extraction. I have been using this product for more than 8 years. The instructions are straightforward and the kit does a fantastic job. I like that elution volume can be as low as 7 microliters. This is relevant for some of my ... read more

We have a 24-slot, 1.5 mL tube holding rotor in this centrifuge. This is not a temperature controlled centrifuge. We have been using this centrifuge for over 5 years and never had a hardware problem. We did experience errors (2-3 times) over this time period but they got resolved after restarting ... read more