Fluorescence imaging is one of our most powerful tools for the study of molecular biology and biochemistry, whether the technique in question is epifluorescence microscopy (excitation and observation of fluorescence from above the specimen); more complex confocal microscopy, which provides a clean, 3-D image; or fluorescence-lifetime imaging microscopy (FLIM), in which the lifetime of the fluorophore signal is measured.
Considerations when choosing a Fluorescence Imaging System:
Key elements of all fluorescence imaging systems include a light source, such as a xenon or mercury lamp, excitation and emission filters, and a dichroic mirror or beamsplitter. Sensitive detection of biological molecules is a major benefit of the technique, as is multicolor detection, which eliminates sample-to-sample variation and is both time and cost effective.
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- Whole Brain Imaging
Miltenyi Biotec
We as a research institute trying to understand the underlying structure and processes of brains did choose the Ultramicroscope II because it was the only suitable light sheet microscope that was ... Read Review - Robust and Innovative LSFM for Large Samples
Miltenyi Biotec
We are using Miltenyi (former LaVision Biotec) Light Sheet Fluorescence Microscopes (LSFM) for more than 10 years now. In our environment (preclinical drug development) we particularly value its high ... Read Review - Powerful Imaging Microscope, but Not Easy to Learn
Molecular Devices, LLC
We acquired this microscope to fulfill several purposes, including cell counting and climate controlled expriments using both blood leukocytes (neutrophils, monocytes) and epithelial cells. Specific ... Read Review