June 11, 2012 - Thermo Fisher Scientific Inc. is pleased to announce a quick and efficient method to determine hesperidin in orange peel and other citrus fruits. Application Brief 142: Rapid Determination of Hesperidin in Orange Peel Using Accelerated Solvent Extraction and UHPLC demonstrates extraction of an orange peel sample in 35 minutes and separation in 4 minutes with only 40 mL solvent consumed for each extraction. An automated accelerated solvent extraction system uses high temperature and pressure to perform quick sample extractions while a rapid separation liquid chromatography (RSLC) system with an RSLC 120 C18 column separates the samples.
Hesperidin is a strong antioxidant reported to be beneficial to good health. Therefore, this new approach will be of interest to companies that purify and sell natural products for pharmaceutical uses. Compared to the Soxhlet approach, this automated extraction method saves significant time and solvent with similar result.
This application note and many others can be found at www.thermoscientific.com/dionex under the Documents tab.
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