Filippo Massa

Filippo Massa

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Articles by Filippo Massa

  • Ready to Use TAE Powder

    Wednesday, June 28, 2023
    Little pouches pre-weighted to obtain 1 liter of 1x TAE buffer. They are very useful for our group that performs routinely a lot of agarose electrophoresis and they avoid the complains about empty bottle of buffer... read more
  • Excellent for Small Volume Sonication

    Tuesday, April 04, 2023
    This is a small vessel that we used for small volume sonication with the Covaris focused sonicator S220. Very useful when dealing with small amounts of starting material (50-100K nuclei) and you can't afford large volumes for sonication as for the bigger 1 mL tubes. The fiber included in the tubes ... read more
  • Essential with Covaris Sonicators

    Friday, March 31, 2023
    Essential consumable to use with the Covaris Focused Sonication systems. We used it with the S220 machine and they performed excellently. The fiber included in the tube drastically increases the sonication efficiency compare to the normal tubes where the liquid absorbs much of the wave force by ... read more
  • Best Loading Dye for DNA Electrophoresis

    Friday, March 03, 2023
    Excellent loading buffer for DNA electrophoresis, with the advantage that this dye doesn't absorb the UV and doesn't present the usual dark shadow around 500bp typical in other loading dye (very annoying when the fragment is around that size). read more
  • Sharp 100bp Ladder

    Thursday, March 02, 2023
    Very good DNA ladder for 100bp bands. Good resolution and consistency between the bands, with brighter 500bp and 1000bp bands as reference. NEB provides a better loading buffer (violet) that doesn't present the shadow around 500bp, present in other loading dyes, when using the UV. read more
  • Excellent Columns with Very Low Elution Volume

    Wednesday, February 22, 2023
    Very good kit for minipreps. It has comparable yield with the more expensive columns from main suppliers, but NEB has also the advantage of the proprietary funnel-shaped filter, allowing small volumes for the full elution. read more
  • Benchtop Mini Centrifuge

    Friday, February 17, 2023
    Very useful small, bench-top centrifuge to spin down samples. It is very flexible because it is equipped with two rotors for 1.5 mL tubes or 2 strips (8 wells) and the adapter to fit 0.5 or 0.2 mL tubes as well. The use is simple and the security lock is quiet solid. read more
  • Excellent Performance, Consumables Are a Bit Expensive

    Thursday, February 16, 2023
    Excellent sonicator for chromatin fragmentation before ChIP experiments. The temperature controlled water bath and the focused sonication system are perfect to maintain the samples at constant temperature and deliver a smaller amount of energy to achieve correct fragmentation compared with other ... read more
  • DNA Concentration and Purification in Small Volumes

    Wednesday, February 15, 2023
    Excellent columns to purify DNA from both PCR reactions (primers elimination) or digested plasmids (enzymes elimination). These columns perform as well as the more expensive ones from main suppliers, but their peculiar and proprietary funnel shape allow the elution in much lower volume (down to 6 µl... read more
  • Good, but Not Optimal for Large Proteins Purification

    Thursday, February 02, 2023
    Affordable and reliable resin for the purification of His-tagged protein. The buffers are detailed in the datasheet and easy to prepare. Nevertheless, the yield for large proteins is not optimal, and a relatively high amount of protein is still found in the flowthrough: we purified 180 KDa protein (... read more
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