Filippo Massa

Filippo Massa

PostDoc

Articles by Filippo Massa

  • Perfect for Elisa Plates Shaking, but No System to Fix the Plates

    Tuesday, July 16, 2024
    Excellent to host 4 ELISA plates with orbital shaking, but you can scale up if you pile them. The structure is very solid and the analogic control (knots) are largely enough to do the job to modulate speed and eventually, the time. Small limitation: the stirrer doesn't have a lock to dock and ... read more
  • Excellent Core Unit for the Nucleofactor Electroporation System

    Tuesday, July 16, 2024
    Excellent core unit for electroporation ! We used an ancient Lonza electroporator system Amaxa for primary cells. After upgrading to this new core, everything began to go smooth much smoother. We are now using this system routinely for the electroporation of different cell types: difficult-to -... read more
  • Flexible Electroporation Unit for the 4D-Nucleofector System

    Tuesday, July 16, 2024
    Essential unit to couple with the Nucleofactor Core unit. It can host two different vessel sizes, 8 wells strips (with the advantage that the wells can be used independently) and 100ul cuvettes. Very flexible, it allowed use to reduced the reactive for expensive experiments (20ul volume for CRISPR/ ... read more
  • Bright and Strong Protein Staining

    Monday, April 08, 2024
    Brilliant Blue R-250 powder to prepare Coomassie staining buffer. We used it by routine to validate the purity and integrity of purified proteins. We dissolve 0,01% of the powder in 40% Met-OH, 10% Acetic acid buffer for couple of hours before filtering. When freshly prepared, the Coomassi fully ... read more
  • Perfect ELISPOT Assay Plate for Few Samples

    Tuesday, April 02, 2024
    We perform a lot of Elispot assays using human PBMC. Since we use patients derived samples, we process few samples each experiment. This 8-wells independent strips are very useful compared with the full plate Elispot and allow us to use only the wells we need, without opening an entire plate. read more
  • Reliable PVDF Elispot Plates

    Tuesday, April 02, 2024
    Excellent PVDF plates for large scale Elispot assays. Fast incubation with 35% Et-OH is sufficient to activate the membrane. These plates are very useful for large scale analysis of IFg secreting cells from in vivo experiments. The signal/background signal is very strong when using PNPP substrate. read more
  • Good Spinner Flask for Suspension Cells, but Easily Contaminated

    Tuesday, April 02, 2024
    Good flask for secreted proteins production with suspension cells. The system is stirring very well, but the porous coating of the magnet is not excellent, since it is very prone to rusty and/or housing of contaminant microorganisms even after autoclave. We replaced the magnet with a better (mostly ... read more
  • Affordable Cell Strainer

    Tuesday, April 02, 2024
    We perform a lot of cell isolation from dissociated tissue (mouse spleen). We therefor require single cell suspension before the purification step. This filter, fitting the 50ml tubes, is much more convenient compared with the costly major brands, still performing excellently the last filtration of... read more
  • Ready to Use Kanamycine

    Friday, January 26, 2024
    We perform a lot of cloning, so we need a lot of Kanamycine for the bacteria selection and growth. This formulation is already at classical concentration to be readily diluted 1:500 to 1:1000 according to your needs. The solution is also very stable and can be kept at 4°C for many weeks (we went up... read more
  • Magnets for Eppendorf Tubes

    Friday, January 26, 2024
    Magnetic beads based protein purification can be very tricky with small volumes. This magnet can arrange up to 6 Eppendorf tubes (1,5 and 2 ml), allowing the process of different samples at the same time. The magnet is very strong and the attraction of the beads takes only a few minutes and is very ... read more
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