Saurat Dione

Saurat Dione

Engineer

Articles by Saurat Dione

  • White DMEM

    Wednesday, May 08, 2024
    I have been using this DMEM for the fibroblasts culture because it has been reported that phenol red can cause disturbances afterwards during the assays. All I had to add was antibiotics (Penicillin/Streptomycin) and foetal bovine serum. As far as I’m concerned, for this study, I had fibroblasts ... read more
  • QIAquick Gel Extraction Kit

    Wednesday, January 17, 2024
    I have been using this gel extraction kit a few times already to sequence amplified DNA bands, which run over agarose gel. This kit is very easy to use, and instructions are made very clear regarding the reagents included in the kit. I recommend this QIAquick Gel Extraction Kit! read more
  • Alizarin Red Staining

    Wednesday, January 17, 2024
    Over the last few months, I have been using this Alizarin Red S for the staining of my cells. Indeed, the Alizarin Red S is going to link itself to the calcium, which allows us to observe the extracellular matrix. As I work on healthy osteoblasts as well as osteoblasts from patients’ biopsies, it ... read more
  • Western Blot Transfer Solution

    Wednesday, January 17, 2024
    I have been using this buffer solution for the transfer stage during my western blot. I have to say that I would not use another because, first of all, it is very easy to prepare as we just need to add ethanol. Second of all, this packaging comes with a little tap which makes it easy to pour. ... read more
  • Dry Bath Incubator

    Wednesday, January 10, 2024
    I use this dry bath incubator daily for various experiments that require sample heating, such as Western Blotting. All I can say is that this dry bath incubator works very well, has a rapid heating rate, and is very stable. I highly recommend it! read more
  • Easy to Use Portable Pipet-Aid

    Wednesday, January 10, 2024
    In our laboratory, we use this pipet-aid daily for various experiments. Indeed, every research has its own and it is time-saving as it allows us to pipet a large volume. Moreover, this XPress model is very easy to use, there has been no leak so far, and we have the possibility of adjusting the speed... read more
  • Synth-a-Freeze Cryopreservation Medium

    Monday, January 08, 2024
    I have been using the Synth-a-Freeze cryopreservation medium to freeze my mesenchymal stem cells and my PBMCs. This chemical solvent is very easy to use, already containing 10% of DMSO and it has proven efficient in terms of improving cell viability after thawing. read more
  • Poly-L-lysine Solution

    Monday, January 08, 2024
    I have been using the Poly-L-lysine solution during my cell culture experiments. I use adherent cells (HEK) but sometimes, I have to incubate them with specific mediums for different incubation times (sometimes overnight). Sometimes, depending on the medium used, a lot of my cells detach overnight ... read more
  • Trypan Blue Solution

    Monday, January 08, 2024
    I have been using this stain solution very regularly while doing my culture cell experiments. Indeed, when I need to count cells, I automatically use the Trypan Blue Solution, which allows me to quickly identify the viable cells. I highly recommend it ! read more
  • Lab Tek Chambered Coverglass

    Monday, January 08, 2024
    I used these chambered Coverglass for my immunofluorescence experiments, which was time-saving because I could do the cell culture of my cell directly onto the coverslip. Even though the coverslip has the correct thickness, and it is very easy to use, I find it very fragile as it broke several times... read more
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