Saurat Dione

Saurat Dione

Engineer

Articles by Saurat Dione

  • Western Blot Transfer Solution

    Wednesday, January 17, 2024
    I have been using this buffer solution for the transfer stage during my western blot. I have to say that I would not use another because, first of all, it is very easy to prepare as we just need to add ethanol. Second of all, this packaging comes with a little tap which makes it easy to pour. ... read more
  • Alizarin Red Staining

    Wednesday, January 17, 2024
    Over the last few months, I have been using this Alizarin Red S for the staining of my cells. Indeed, the Alizarin Red S is going to link itself to the calcium, which allows us to observe the extracellular matrix. As I work on healthy osteoblasts as well as osteoblasts from patients’ biopsies, it ... read more
  • QIAquick Gel Extraction Kit

    Wednesday, January 17, 2024
    I have been using this gel extraction kit a few times already to sequence amplified DNA bands, which run over agarose gel. This kit is very easy to use, and instructions are made very clear regarding the reagents included in the kit. I recommend this QIAquick Gel Extraction Kit! read more
  • Easy to Use Portable Pipet-Aid

    Wednesday, January 10, 2024
    In our laboratory, we use this pipet-aid daily for various experiments. Indeed, every research has its own and it is time-saving as it allows us to pipet a large volume. Moreover, this XPress model is very easy to use, there has been no leak so far, and we have the possibility of adjusting the speed... read more
  • Dry Bath Incubator

    Wednesday, January 10, 2024
    I use this dry bath incubator daily for various experiments that require sample heating, such as Western Blotting. All I can say is that this dry bath incubator works very well, has a rapid heating rate, and is very stable. I highly recommend it! read more
  • Real-Time PCR System

    Monday, January 08, 2024
    I regularly use this Real-Time PCR System and I have to say that I really like it. I find it very easy to use, with an intuitive interface and it also offers an intuitive data analysis setting. So far, this system delivered accurate results, which are easily reproducible. However, I find it ... read more
  • Centrifugal Filters for Protein Purification

    Monday, January 08, 2024
    I have been using these centrifugal filters for a while now to purify my samples. These Amicon Ultra are very easy to use, can put up to 500 µl of samples in the filter and it can easily fit inside the Eppendorf Microcentrifuge. As far as I can tell, every test I have performed on these purified ... read more
  • Cell Culture Media – MEM a

    Monday, January 08, 2024
    I have been using this MEMa media to differentiate monocytes into osteoclasts. In order to obtain a successful differentiation, I have to add antibiotics, Hyclone serum, B-Mercapto, RANKL, and MCSF to the media. So far, I have used this media every time and I can say that the cell viability was ... read more
  • Mini-PROTEAN TGX Gels

    Monday, January 08, 2024
    Our lab has been using these precast gels quite often, which is time-saving because the gel preparation is a long process. Moreover, these gels go from 4% to 15%, which allows the separation of proteins between 5 and 200 kDa. It is possible to have a run time as short as 15 minutes with these gels ... read more
  • Power Pack for Gel Electrophoresis

    Monday, January 08, 2024
    I have been using this power pack to run my electrophoresis gel for a while. It is very easy to use and it works well, I have had no problems with the migration of my proteins on the gel. However, this device is very sensitive to the cabling and leaks within the gel support, which leads to an error ... read more
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