I typically use this resin when my recombinant His-tagged protein purification process requires DTT and EDTA in my binding buffer. This resin is different from the traditional Ni-NTA Agarose resins in that imidazole concentration for the wash and elution steps is low (1-5 mM for the wash step and 25... read more

I have been using this centrifugal filter unit for more than 15 years. I typically use this unit for my recombinant protein purification process during the sample concentration step. Typical volumes that I work on are between 10-30 mL and my protein sizes >100 kDa. Easy to use and the best product ... read more

I used this kit for 96-well format at 25 µL of XTT reconstituted solution per well containing 100 µL of medium. The incubation period was 3 h. The kit is easy to use and I followed the manufacturer's instructions with reproducible results. read more

I have used this kit for purification of CRISPR sgRNAs. sgRNAs are transcribed in vitro and the purification is done with a slight modification that is compatible for small RNA purification. I typically get yields of 50-100 µg with high purity and they work really well for CRISPR RNP experiments. read more

I prefer these gels when I need to resolve multiple proteins of varying sizes on the same gel. I work with a protein complex that contains three proteins of ~300 kDa, ~60 kDa, and ~20 kDa. This gel beautifully resolves all three proteins and even the large sized protein is efficiently transferred to... read more