I use vacuum lines hooked up to glass flasks to aspirate supernatants from pelleted bacterial and mammalian cells. The Millex-FH Filter does a good job by serving as the vacuum line protector. These last for few months. read more

This ready-to-use reagent from Thermo Fisher Scientific can be used as a sheath fluid in flow cytometry systems. I work with Sony Cell Sorter SH800S and have been using this reagent for more than 3 years. read more

I prefer these gels when I need to resolve multiple proteins of varying sizes on the same gel. I work with a protein complex that contains three proteins of ~300 kDa, ~60 kDa, and ~20 kDa. This gel beautifully resolves all three proteins and even the large sized protein is efficiently transferred to... read more

I have used this kit for purification of CRISPR sgRNAs. sgRNAs are transcribed in vitro and the purification is done with a slight modification that is compatible for small RNA purification. I typically get yields of 50-100 µg with high purity and they work really well for CRISPR RNP experiments. read more

I used this kit for 96-well format at 25 µL of XTT reconstituted solution per well containing 100 µL of medium. The incubation period was 3 h. The kit is easy to use and I followed the manufacturer's instructions with reproducible results. read more