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Buffer Set for Flowcytometry-Based Assays

Parijat Sarkar
I used the cyto-fast buffers to stain cells for flow cytometry. The buffers offer a quick alternative for fixing and permeabilizing cells in suspension for FACS. The buffers ... read more
Buffer for Immunofluorescent Staining

Parijat Sarkar
I use this cell staining buffer to resuspend cells for flow cytometry. I have found that cells are stable in this buffer for 2-3 hours on ice. The buffer is ready to use and ... read more
Buffer for Immunofluorescent Staining of Nuclear Proteins

Parijat Sarkar
I used this kit to fix and label a nuclear protein (transcription factor). The permeabilization buffer provided with the kit allows efficient nuclear membrane permeabilization... read more
Trizol Reagent for Effective RNA Isolation

Parijat Sarkar
I use the Trizol reagent for RNA isolation from mammalian cells. I always get high yield of RNA for my RT-PCR experiments when I use this product. I have also used this ... read more
Mini Spin Columns for DNA Isolation

Parijat Sarkar
I have used these columns for plasmid mini-preps. I always get >20µg yield from 5 ml overnight cultures. The columns can carry approximately 900 ul solutions which is perfect ... read more
Yeast Protein Extraction Reagent

Parijat Sarkar
I use this extraction reagent for a range of downstream applications, including Western blotting, enzyme assays, and protein analysis. Its efficiency in lysing yeast cells is ... read more
Malassez Counting Chambers

Laetitia CLOTAIRE
I have used the Marienfeld Superior counting chambers as counting instruments to determine the number of cells present in my samples. This allows me to configure my ... read more
Filter for Huge Volumes

Laetitia CLOTAIRE
I use this sterile bottle vacuum filter (0.22 µm pores) to filter my solutions specific to cell culture. In order to avoid artifacts due to the presence of particles, I ... read more
Trypsin for Cell Dissociation

Laetitia CLOTAIRE
Usually, I detached my cells by using this sterile trypsin-EDTA mixture solution (0.05%). This works easier for HEK-293, CHO, HK-2, THP-1 cells by it’s more difficult for ... read more
Master Mix for qPCR

Parijat Sarkar
I have found the PowerTrack SYBR Green Master Mix to be a useful alternative in my real-time PCR experiments. The inclusion of an inert blue dye enhances visibility without ... read more
BCA Protein Assay Kit

Parijat Sarkar
For measuring protein concentration in cell lysates, I use the BCA kit. The BCA kit has proven to be an indispensable tool in my Western blot loading for accurate protein ... read more
Cloning Kit with Key Specifications

Neha Mittal
The cloning of the specific gene or genes needs key specified chemicals, and reagents. For this purpose, the zero blunt cloning kit by Invitrogen is a great choice, and it ... read more
Good Quality Reagent Starter Kit for Initial Experiments

Neha Mittal
The TaqMan kit is always a good option for real-time PCR experiments. However, the Applied Biosystems kit is a good option if you would like to perform the standardizations of... read more
Amazing Nuclease Free Solution

Neha Mittal
In molecular biology experiments to perform the key downstream analysis it is sometimes highly required for your sample or extracted nucleic acids to be cleaned with other ... read more
Excellent Quality Molecular Grade Water

Neha Mittal
To prepare the PCR primers and other reagents we need to use Nuclease Free water which keeps the environment of the prepared solutions clean and contamination free. For this ... read more