Lenin Veeraval

Mini Centrifuge for Sample Spin Down and Microfiltration
Monday, June 13, 2022
The mini centrifuge is very essential equipment for any researcher on his bench to quickly spin down small volume DNA and antibody solutions from the wall and cap of the microcentrifuge tube or epitube. Vortex and quick spin are two of the most frequent activities in immunochemistry and ... read more
Hybridisation Oven for DNA, RNA and Protein Hybridisation Experiments
Friday, June 10, 2022
The Ratek Hybridisation Oven is handy bio-equipment used for experiments like southern blot, northern blot and western blot for the hybridization of DNA, RNA and proteins respectively in a membrane. Specific temperature can be regulated for a specific hybridization. This Ratek oven has got ... read more
Lipofectamine for Transfecting Plasmid DNA in Cell Lines and Primary Hippocampal Neurons
Thursday, June 09, 2022
We use Lipofectamine 2000 to transfect plasmid DNA to silence our protein of interest in human colorectal cancer cell line Caco2, HEK, primary hippocampal neurons and also ependymal cells. Lipid DNA complex is made by diluting DNA and lipofectamine separately in optiMEM media and mixing them in the ... read more
Isolation of Purified Plasmid DNA for Transfection and Molecular Cloning
Wednesday, June 08, 2022
The Qiagen plasmid kit is a very handy plasmid purification kit for molecular biologists. Usage of toxic chemicals such as phenol and chloroform can be avoided using this kit. It uses anion exchange resin for purifying transfection grade plasmid DNA obtained from bacterial lysates. Up to 500 µg of ... read more
Thermo Scientific Kit for Nuclear and Cytoplasmic Protein Extraction
Tuesday, June 07, 2022
This Thermo Scientific Nuclear and Cytoplasmic Extraction Kit is used for extracting nuclear proteins and cytoplasmic proteins separately without getting mixed. Density gradient centrifugation is one of the commonly used methods to separate nuclear content from cytoplasm, however it involves lengthy... read more
Biosafety Cabinet for Cell Culture Facility
Monday, June 06, 2022
The NordicSafe Class II Biosafety Cabinets provide a great aseptic environment for mammalian tissue/cell culture activities. The inward airflow and partially enclosed shutter in this NordicSafe cabinet provides protection for both the user and the samples (from getting contamination). It's less ... read more
Tris Acetate Precast Gel - Large Molecular Weight Protein Separation
Friday, June 03, 2022
This Invitrogen Tris-Acetate precast gel is SDS - polyacrylamide gel that can be used for separating large molecular weight proteins between 400 kDa to 60 kDa, whereas Tris-Glycine gels can resolve proteins less than 200 kDa. Apart from this specific usage, like other NuPage precast gel, Tris-... read more
Sliding Microtome for Hard and Large Specimens
Thursday, June 02, 2022
The sliding microtome is a histology sectioning instrument alternative to the rotatory microtome. Unlike the rotatory microtome, the sliding type provides a stage for holding larger size tissues (e.g. biopsies from human tissues). Like rotatory type, sliding type also processes paraffin embedded ... read more
Fibre Optic Light Source for Animal Dissection and Tissue Sectioning
Wednesday, June 01, 2022
An optimal light source plays a crucial role in the microdissection of brain areas. Light intensity demarcates tissue thickness and integrity which is varied in different areas of brain. Hence, by using an appropriate light source we can perform dissection of brain areas, say, the hippocampus or ... read more
Vortex for Cell and Molecular Biology Experiments
Tuesday, May 31, 2022
The Vortex 3, originally manufactured by IKA and supplied by Merck, is used for cell and molecular biology experiments like any other vortex. Used for mixing antibody solutions for immunochemistry and also for mixing any diluted solutions. The vortex comes with adaptors to fit epitopes, 250mL flasks... read more