Lenin Veeraval

Lenin Veeraval

Graduate Student

Articles by Lenin Veeraval

  • Glass-bottomed 6-well Plate for Ependymal Cell Culture

    Wednesday, May 25, 2022
    MatTek glass-bottomed culture plates are used for ependymal cells cultures. Since they are an epithelial type of cells, the cells need to be seeded closely, which is facilitated by this glass-bottomed 6-well dish. This dish is also a user friendly alternative for performing immunofluorescence, which... read more

  • Floating Bath for Microtome Sectioning

    Tuesday, May 24, 2022
    This Thermoline floating bath is used in microtome assisted paraffin sectioning for unrolling and unwrinkling the sections, which occurs during sectioning. At least seven to eight paraffin ribbons containing 7-10 sections can be allowed to float at a time in this 7 liter floating bath. For long ... read more

  • Cryotomy Tissue Embedding Compound

    Friday, May 20, 2022
    This frozen section media from Leica Biosystems is used for embedding tissues for frozen/cryo sectioning with a cryotome. Before embedding in freezing media the tissue should be processed in sucrose solution with increasing concentration 15% to 30%. This prevents the tissue from freeze damaging. ... read more

  • PVDF for Western Blot Transfer

    Thursday, May 19, 2022
    PVDF is a good membrane for transferring and immobilising both cytoplasmic and nuclear proteins from SDS-PAGE gel. Infrared dyes used for proteins visualization are captured well in PVDF membrane. Activation of PVDF membrane is required before protein transfer by methanol for 1 min and MilliQ water ... read more

  • Mr. Frosty for Cryopreserving Your Expanded Cells

    Wednesday, May 18, 2022
    The Mr. Frosty cryopreservation container is used to cryopreserve cells in liquid nitrogen for long term storage. Placing the cryotube containing cells in the freezing container allows the cells to freeze at -1 degree per minute which is the optimal rate for preserving cells. This container with a ... read more

  • Coating Substance to Culture Stem Cells and Neurons

    Tuesday, May 17, 2022
    Poly-L-lysine (PLL) hydrobromide is an amino-acid/coating substrate used to promote cell adhesion in stem cell and neuronal culture. For stem cell culture 1% of 10mg/mL PLL stock is recommended for coating dishes. Same concentration is used for neuronal cultures, in addition to Poly-L-ornithine. read more

  • Histology Microscope Coverglass

    Monday, May 16, 2022
    The Hurst coverglass is a widely used cover for immunostained or dye stained histological sections on a microscopic slide for imaging purposes. This coverglass helps to flatten the section very well, reduces the rate of evaporation and reduced photobleaching with the use of proper mountant. Needs to... read more

  • Membrane Filter for Filtering Sterilized Water

    Friday, May 13, 2022
    These Millipak filters are used to replace saturated filters from the Merck Milli-Q water purification system. This Millipak filters particulates and microbes that are up to 0.2 micron in size. Unlike previous versions, release of filtered purified water from this filter is slightly faster. Apart ... read more

  • Paraffin Tissue Embedding Platform

    Thursday, May 12, 2022
    The TES Valida Paraffin Embedding Centre is handy user friendly equipment that provides a platform for tissue embedding in paraffin. This centre has three work stations: first is a paraffin reservoir tank, second is the paraffin dispensing and embedding platform and finally the solidification/... read more

  • Nice Dispenser - Less Benchwork Fatigue

    Wednesday, May 11, 2022
    This Thermo Scientific dispenser is very good for dispensing liquids in large volume. Very helpful especially while working in a sterile environment. Controlled uptake and release compared to other dispensers available in the market. Pretty stable for surface disinfection with 70% alcohol. read more
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