Filippo Massa

Filippo Massa

PostDoc

Articles by Filippo Massa

  • Bright and Strong Protein Staining

    Monday, April 08, 2024
    Brilliant Blue R-250 powder to prepare Coomassie staining buffer. We used it by routine to validate the purity and integrity of purified proteins. We dissolve 0,01% of the powder in 40% Met-OH, 10% Acetic acid buffer for couple of hours before filtering. When freshly prepared, the Coomassi fully ... read more
  • Affordable Cell Strainer

    Tuesday, April 02, 2024
    We perform a lot of cell isolation from dissociated tissue (mouse spleen). We therefor require single cell suspension before the purification step. This filter, fitting the 50ml tubes, is much more convenient compared with the costly major brands, still performing excellently the last filtration of... read more
  • Good Spinner Flask for Suspension Cells, but Easily Contaminated

    Tuesday, April 02, 2024
    Good flask for secreted proteins production with suspension cells. The system is stirring very well, but the porous coating of the magnet is not excellent, since it is very prone to rusty and/or housing of contaminant microorganisms even after autoclave. We replaced the magnet with a better (mostly ... read more
  • Reliable PVDF Elispot Plates

    Tuesday, April 02, 2024
    Excellent PVDF plates for large scale Elispot assays. Fast incubation with 35% Et-OH is sufficient to activate the membrane. These plates are very useful for large scale analysis of IFg secreting cells from in vivo experiments. The signal/background signal is very strong when using PNPP substrate. read more
  • Perfect ELISPOT Assay Plate for Few Samples

    Tuesday, April 02, 2024
    We perform a lot of Elispot assays using human PBMC. Since we use patients derived samples, we process few samples each experiment. This 8-wells independent strips are very useful compared with the full plate Elispot and allow us to use only the wells we need, without opening an entire plate. read more
  • Excellent Antibiotic for Bacteria Selection

    Friday, January 26, 2024
    Performing a lot of cloning, we tested a lot of antibiotics. This reference of Ampicillin works very well and it is often in offer, with nice reductions. Budget restrictions made it our primary choice for ampicillin. The powder is easily dissolved and the aliquoted solution is very stable. read more
  • Magnets for Eppendorf Tubes

    Friday, January 26, 2024
    Magnetic beads based protein purification can be very tricky with small volumes. This magnet can arrange up to 6 Eppendorf tubes (1,5 and 2 ml), allowing the process of different samples at the same time. The magnet is very strong and the attraction of the beads takes only a few minutes and is very ... read more
  • Ready to Use Kanamycine

    Friday, January 26, 2024
    We perform a lot of cloning, so we need a lot of Kanamycine for the bacteria selection and growth. This formulation is already at classical concentration to be readily diluted 1:500 to 1:1000 according to your needs. The solution is also very stable and can be kept at 4°C for many weeks (we went up... read more
  • Stirrer That Is Not Good for Use in Cell Incubators

    Wednesday, January 17, 2024
    We needed a compact stirrer to fit in warm incubators (standard cell culture conditions). We purchase this model for its reduced size and the relatively low price. The stirrer is very light and it works fine for normal purposes at room temperature, but it broke down the first time we tried it in the... read more
  • Fast and Complete Kit for Proteins Quantification

    Wednesday, January 17, 2024
    Excellent kit for the quantification of the protein. Required volumes can be scaled to increase sensibility or save precious samples. The kit contains two different solutions to mix before incubation and 10 ultra-clean BSA glass ampules. the reactions are set very rapidly and they can become even ... read more
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