Laetitia CLOTAIRE

Laetitia CLOTAIRE

Ph.D student

Articles by Laetitia CLOTAIRE

  • Protein Ladder

    Friday, December 08, 2023
    I highly recommend this protein ladder for your Western blot because it’s practical to use and to look at real time the migration of your samples in the gel. You can see rapidly if there are any problems. It’s really helpful all along the western blot process to avoid technical problems. read more
  • Eppendorf Centrifuge

    Friday, December 08, 2023
    My laboratory recently purchased this equipment. It's very well thought-out, as you can use several types of samples (microtubes, plates, flacon…) on the same rotor. There are numerous accessories for adapting samples. What's more, it's possible to add centrifugation programs. You can change the ... read more
  • pH / Ion Meter

    Thursday, December 07, 2023
    This pH meter is well designed and very easy to use. The pH measurement is precise and reproducible. You will have quick results. It is small and light and so easy to carry with you to another laboratory. The calibration is also easy to do. Be careful with the probe (fragile even if there is ... read more
  • ThermoMixer

    Thursday, December 07, 2023
    The compact Thermomixer allows mixing the samples at low speed (300 RPM) or high speed (1,400 RPM) but also controls the temperature (4°C to 100°C). It is used quite often for long mixtures time but also as a heater before certain manipulation. It is necessary to preheat it for less than 10 min to ... read more
  • Magnetic Beads for Immunopurification

    Wednesday, November 29, 2023
    I've been using these anti-HA magnetic microbeads (size 1µM) for a few years now. After preparing my cell extracts, I put my lysates in the presence of these beads to extract the proteins of interest. It's a relatively rapid process requiring 3 steps. The first is a rinsing step, which requires the ... read more
  • Magnetic Separation Rack for 1.5mL Microtubes

    Wednesday, November 29, 2023
    In our team, this rack is an essential tool for immunopurification manipulations. It has several advantages: it can take 6 1.5mL microtubes simultaneously, and the magnet is located on one edge of the microtube. The biggest advantage is that you can see the samples being taken at the same time as ... read more
  • Lipofectamine 3000 Reagent

    Wednesday, November 29, 2023
    This kit is a little expensive, but this is justified by the quantity of transfections possible with 750µL (around 7 manipulations). It's a simple method for transfecting stable cell lines (HEK 293, COS-7, HK2, etc). In 7 quick steps, your cells are transfected. A user guide is provided, enabling ... read more
  • Water Stabilizer

    Tuesday, November 28, 2023
    In our laboratory, we only use rea-stabil in the cell culture room to stabilize the water in thermal baths and incubators. This stabilizer is sufficiently concentrated to be used at a rate of one cup per 1 liter. It prevents the formation of bacteria or any other contamination. You can keep it for a... read more
  • Cell Imaging & Microscopy

    Wednesday, November 15, 2023
    Our laboratory uses this camera for routine tasks. We are checking and counting our cells with this microscopy camera. We are taking pictures of our cell with highly accurate color rendition thanks to the full 4K UHD resolution and a frame rate of 30 fps. It’s really easy to use and I have never had... read more
  • Useful Water Bath

    Monday, November 13, 2023
    I use this bath during the western blot process or during any experimentation that needs to warm previously frozen samples. You can calibrate the temperature starting from 10°C to 99°C. The only negative point is the fact that if you want to increase the sample temperature quickly you need a bit of ... read more
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