Usually, I detached my cells by using this sterile trypsin-EDTA mixture solution (0.05%). This works easier for HEK-293, CHO, HK-2, THP-1 cells by it’s more difficult for mesenchymal stells cells and fibroblasts. I recommend this product because its quickly effective and during cell dissociation its... read more

I use the Immobilon Western HRP Substrate during the western blot detection stage. You need to prepare some before detection at a ratio of 1:1 of peroxide solution and luminol reagent. Be careful when you use them to avoid contamination. It's not expensive and easy to use. read more

During the western blot transfer stage, I use these pre-cut fiber transfer pads. Using these thick papers (1.5 mm thick) allows the sandwiches to be tight and prevents the membrane from moving. With one pad, you can cut it out to use it on a sandwich. It’s easy to use and cheap. I highly recommended... read more

During my transfection experiments, I use Opti-MEM (minimum essential medium) culture medium. It is recommended when you are using Lipofectamine reagent (cationic lipids). It's a simple, improved culture medium (substitutes fetal calf serum) that allows the cell (Sp2, AE-1, CHO, BHK-21 and HEK-293) ... read more

I use this oil when I take pictures with microscopy photography in high objective (60X and 100X). This oil is necessary to avoid breaking the lenses and to obtain a clear image. It has a very oily and thick exterior texture, which gradually leads to stains on the edges of the bottle. A little ... read more