Laetitia CLOTAIRE

Laetitia CLOTAIRE

Ph.D student

Articles by Laetitia CLOTAIRE

  • DMEM F-12 for MSC

    Monday, November 13, 2023
    Recently, I used this medium for the maintenance of Mesenchymal stem cells. It’s a classic medium but to use it on MSC you need to complete the medium with a lot of extra (vitamin C, beta-mercapto, SVF, pen/strep, glutamax, and more). Other than that, I never have problems with this medium. I fully ... read more
  • PARR System For Extraction

    Monday, November 13, 2023
    I use this pressure vessel to prepare my membrane extractions. It's a simple system with several pressure control valves. You put your cell suspensions in High Pressure from 10 to 200 bar and by the nitrogen decompression method, you can obtain your protein extraction after more or less 30 minutes, ... read more
  • DMSO for Cell Freezing

    Monday, November 13, 2023
    DMSO (Dimethyl sulfoxide) is widely used for freezing cells and pharmacological molecule preparation. I use it with FBS (Foetal bovin serum) with 10% DMSO which avoids cell explosion during the freezing process. It's easy to use in sterile condition and protect the cells during this process with low... read more
  • 0.22µm Filter for Solutions

    Monday, November 13, 2023
    I highly recommend this kind of micro filter. I like the design with the screw for different sizes of syringes (1 mL, 5 mL,10 mL). I'm using this sterile filter for ionic solution preparation and for cell culture. The pore size of the filter is quite small: 0,22 µm. read more
  • Culture Medium DMEM 1X

    Wednesday, November 01, 2023
    I use usually this medium for HEK293 cells. It is quite a complete medium. There is the essential amino acid and vitamin concentration. I just need to add the antibiotics penicillin streptomycin and bovine serum to use it. However, DMEM for cell culture contains the indicator phenol red which is ... read more
  • Single Electrophysiology

    Tuesday, August 01, 2023
    I used this instrument to records single channels currents on purified proteins. It’s a very good systems to understand biophysical properties of proteins. The system has design to simultaneously record four lipid bilayers. It has built to minimized noise recordings at high bandwidth without the ... read more
  • Useful for Protein Quantification

    Tuesday, August 01, 2023
    I highly recommended this machine if you have place, and you are doing a lot of precise protein quantification (UV Pad for DNA and RNA gels and fluorescence stain imaging). You can adjust inside the box the distance between the lamp and your membrane/gel samples. The negative point is the fact that ... read more
  • Transfert Solution

    Tuesday, August 01, 2023
    In our team, we use this 10x Tris/Glycine Buffer in transfer buffer preparation for western blots. It has a good packaging with a useful tap system to take the volume need. When you prepare the solution, you just need to add water and alcohol (ethanol or methanol). read more
  • Mobile-Phase Ion Chromatography

    Monday, July 24, 2023
    I use acetonitrile only in HPLC (high performance liquid chromatography) experiment as a mobile-phase constituent for many samples. It has a low viscosity, high elution power and good miscibility with water. It also plays a major role as a liquid-liquid extraction solvent. read more
  • Nanodrop for DNA, RNA and Protein Measurement

    Monday, July 24, 2023
    I used this instrument to quantify and look the quality of DNA, RNA and protein samples. You can detect if there is contamination in your samples. The advantage of this machine is the use of small volume of samples (1-2µL). For protein dosage, you can use the BCA, Bradford, and Lowry methods that ... read more
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