Rachel Crossland

Rachel Crossland

Research Associate

Articles by Rachel Crossland

  • Quick Start Bovine Serum Albumin Standard Set

    Friday, February 16, 2024
    I have used the Quick Start Bovine Serum Albumin Standard Set for use with Quick Start Bradford protein assay kits. The kit contains a set of 7 protein concentration standards, 0.125–2.0 mg/ml and makes protein quantification in solution simple and efficient. read more
  • Extracellular Vesicle Isolation Using the Exo-spin™ Mini

    Friday, February 16, 2024
    I have used the Exo-spin™ mini for extracellular vesicle (EV) isolation from serum. EVs were successfully isolated using this system and could be visualised using electron microscopy and nanoparticle tracking analysis. They express typical EV markers CD63, CD9 and CD81. read more
  • Extracellular Vesicle Isolation Using The Total Exosome Isolation Reagent

    Friday, February 16, 2024
    I have used the total Exosome Isolation Reagent to isolate extracellular vesicles (EVs) from the cell culture media of chuman chondrocytes. The reagent is very simple to use and doesn't rely on specialized equipment. However, it can be costly due to the volume of reagent required. The isolated EVs ... read more
  • Gene Expression by qRT-PCR Using the SensiFAST™ Probe Hi-ROX One-Step Kit

    Friday, February 16, 2024
    I have used the SensiFAST™ Probe Hi-ROX One-Step Kit for qRT-PCR analysis of gene expression from mesenchymal stromal cells. The kit can be used with all instruments that require a high concentration of the passive reference dye ROX. I have use the kit with TaqMan assays with high success. read more
  • MicroAmp™ Optical Adhesive Film for qRT-PCR plates

    Friday, February 16, 2024
    I use the MicroAmp™ Optical Adhesive Film for sealing 96-well plates in conjunction with the QS3 qRT-PCR instrument. The films are easy to use with pre-perforated edges and completely seal the plates effectively. read more
  • MicroAmp™ Fast Optical 96-Well Reaction Plate for qRT-PCR

    Friday, February 16, 2024
    I use the MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1 mL, for use with the Quantstudio 3 qRT-PCR platform. The plates are fast-compatible and make gene and microRNA qRT-PCR assessment simple, easy and efficient. read more
  • Staining Cell Membranes Using Wheat Germ Agglutinin (WGA)

    Tuesday, February 13, 2024
    I have used the Wheat Germ Agglutinin Alexa Fluor® 488 conjugate (10 µg/ml, Invitrogen) to stain the cell membranes of human chondrocytes. The cells were incubated for 10 minutes in the dark to stain the cell membrane then mounted using Vectashield medium with DAPI for nuclei staining. The cells ... read more
  • Library Preparation for RNASeq Using TruSeq Stranded mRNA

    Tuesday, February 13, 2024
    I have used the TruSeq Stranded mRNA for library preparation for RNA-Seq. Total RNA samples (n=24) were prepared using the ‘TruSeq Stranded mRNA’ kit (Illumina) following manufacturer's instructions, pooled into one library and sequenced on a NextSeq 500 ‘High-Output - 75 cycle’ (Illumina) ... read more
  • Benchtop Centrifugation With the Fisher Midi Centrifuge

    Tuesday, February 13, 2024
    I use the Fisherbrand™ Midi Centrifuge for routine benchtop centrifugation. It includes two rotors: standard 12-place rotor with lid and eight-place strip rotor for four 0.2mL tube strips or 32 single tubes. These are easily interchangeable via a screw mechanism. The product is easy to use, ... read more
  • DNA Decontamination Using DNAZap

    Tuesday, February 13, 2024
    I use DNAZap™ solutions as a nucleic acid degrading reagent to maintain our lab. The product is able to degrade high levels of potential or known contaminating DNA and RNA from surfaces. It's ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge ... read more
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