Rachel Crossland

CD63 Antibody for Characterization of Extracellular Vesicle Marker
Wednesday, August 30, 2023
I have used the BD Pharmingen™ PE Mouse Anti-Human CD63 to assess presence of CD63 on extracellular vesicles (EVs) isolated from human serum. The presence of this marker fulfils one of the requirements of the International Society for Extracellular Vesicles EV characterisation. Alongside CD63, I ... read more
Assessment of Atp Levels Using Celltiter-Glo® 3D Cell Viability Assay
Wednesday, August 30, 2023
The CellTiter-Glo 2.0 Luminescent Cell Viability Assay (Promega, UK) measures total ATP levels produced by metabolically active cells. I used this product to assess ATP levels in chondrocytes treated with extracellular vesicles (EVs). Luminescence measurements were performed on day 5 of cell culture... read more
Isolation of RNA From Extracellular Vesicles Using the Total Exosome RNA & Protein Isolation Kit
Wednesday, August 30, 2023
I have used the Total Exosome RNA & Protein Isolation Kit to isolate total RNA from extracellular vesicles (EVs) derived from human serum and human cells. The kit effectively isolates low-concentration RNA and can also be adapted to isolate protein. I have used the RNA for downstream NanoString ... read more
Precipitation of Extracellular Vesicles From Human Serum
Wednesday, August 16, 2023
I have used this product to precipitate extracellular vesicles from human serum. The choice for this was for a biomarker study. It should be noted that the precipitation reagent does not isolate "pure" exosomes and the precipitated vesicles should be better termed as extracellular vesicles (EVs). ... read more
MACSPlex Extracellular Vesicle Surface Marker Analysis
Friday, August 11, 2023
We used this product to assess extracellular vesicle (EV) surface markers using EVs derived from the serum of patients undergoing treatment. The product allowed us to effectively discriminate markers that were associated with patient outcome following treatment. read more
Concentrating RNA Using Merck Amino Filter Units
Friday, August 11, 2023
We use this product to concentrate RNA down to a small volume. This is useful to us as we can perform multiple RNA elusions, thus recovering the highest yield possible, yet still reduce the final volume down which helps downstream applications where the RNA concentration is crucial. The columns are ... read more